Efficiency of an alkaline, thermostable, detergent compatible, and organic solvent tolerant lipase with hydrolytic potential in biotreatment of wastewater

• Published in: The Science of the total environment 2023-03, Vol.866, p.161066-161066, Article 161066
• Main Authors: Ariaeenejad, Shohreh, Kavousi, Kaveh, Han, Jian-Lin, Ding, Xue-Zhi, Hosseini Salekdeh, Ghasem
• Format: Article
• Language: English
• Subjects: Animals; Biodegradation; Detergents; Hydrogen-Ion Concentration; Hydrolysis; Lipase; Lipase - chemistry; Metagenome; pH stability; Sheep; Solvents - chemistry; Temperature; Thermo-tolerance; Wastewater
• ISSN: 0048-9697; 1879-1026
• DOI: 10.1016/j.scitotenv.2022.161066

Discharging the tannery wastewater into the environment is a serious challenge worldwide due to the release of severe recalcitrant pollutants such as oil compounds and organic materials. The biological treatment through enzymatic hydrolysis is a cheap and eco-friendly method for eliminating fatty substances from wastewater. In this context, lipases can be utilized for bio-treatment of wastewater in multifaceted industrial applications. To overcome the limitations in removing pollutants in the effluent, we aimed to identify a novel robust stable lipase (PersiLipase1) from metagenomic data of tannery wastewater for effective bio-degradation of the oily wastewater pollution. The lipase displayed remarkable thermostability and maintained over 81 % of its activity at 60 °C.After prolonged incubation for 35 days at 60°C, the PersiLipase1 still maintained 53.9 % of its activity. The enzyme also retained over 67 % of its activity in a wide range of pH (4.0 to 9.0). In addition, PersiLipase1 demonstrated considerable tolerance toward metal ions and organic solvents (e.g., retaining >70% activity after the addition of 100 mM of chemicals). Hydrolysis of olive oil and sheep fat by this enzyme showed 100 % efficiency. Furthermore, the PersiLipase1 proved to be efficient for biotreatment of oil and grease from tannery wastewater with the hydrolysis efficiency of 90.76 % ± 0.88. These results demonstrated that the metagenome-derived PersiLipase1 from tannery wastewater has a promising potential for the biodegradation and management of oily wastewater pollution. [Display omitted] •A novel metagenome-derived PersiLipase1 was screened from tannery wastewater.•The PersiLipase1 hydrolyzed animal fat and plant oil sources at a high rate.•The novel Lipase was active and stable under harsh conditions and long storage.•The robust, stable lipase could effectively degrade oily wastewater pollution.