3D printing of a controlled urea delivery device for the prevention of tooth decay

• Published in: International journal of pharmaceutics 2023-01, Vol.631, p.122528-122528, Article 122528
• Main Authors: Berger, Valentine, Green Buzhor, Marina, Evstafeva, Diana, Mügeli, Lena, Leroux, Jean-Christophe
• Format: Article
• Language: English
• Subjects: 3D printing; Carbohydrates; Dental Caries - prevention & control; Drug Liberation; Fluorides; Humans; Personalized medical device; Printing, Three-Dimensional; Tablets; Targeted site release; Technology, Pharmaceutical; Tooth decay; Tunable drug delivery; Urea
• ISSN: 0378-5173; 1873-3476
• DOI: 10.1016/j.ijpharm.2022.122528

[Display omitted] •Fabrication of a personalized 3D printed urea-loaded mouthguard.•Tunable urea release obtained by adjusting the polymer matrix’ composition.•Localized delivery achieved with a unique design.•Buffering of pH drop demonstrated in vitro with Streptococcus salivarius. Dental caries is one of the most widespread chronic infectious diseases in the world. It is mainly caused by the production of acid in the biofilm from the bacterial metabolism of carbohydrates. Nowadays, the prevention of caries is mainly based on the use of topical formulations containing fluoride. However, effective fluoride supplementation may not be sufficient in high-risk individuals, leading to the exploration of alternative strategies such as the neutralization of acid in the oral cavity. Urea is hydrolyzed into ammonia by oral bacteria, leading to a local alkalization that may counteract tooth decay. Herein, we report the fabrication of 3D printed personalized dental trays with a local and prolonged release of urea. Composite filaments with tunable urea release kinetics were produced by hot melt extrusion of poly(ε-caprolactone) and poly(vinyl alcohol) or poly(ethylene glycol) blends mixed with urea. The filaments were further used to 3D print by fused deposition modeling objects capable of releasing urea in a sustained and spatially controlled manner. In vitro studies performed in the presence of Streptococcus salivarius demonstrated the ability of urea released from a 3D printed model toothguards to reduce the pH drop induced by carbohydrates. This study showed the potential of urea-loaded devices to reduce cariogenic acidification of the environment surrounding the enamel by delivering urea directly to the tooth surface.