Effects of methyl jasmonate and NaCl treatments on the resveratrol accumulation and defensive responses in germinated peanut (Arachis hypogaea L.)

• Published in: Plant physiology and biochemistry 2023-01, Vol.194, p.664-673
• Main Authors: Yin, Yongqi, Hu, Jingjing, Yang, Zhengfei, Fang, Weiming, Yang, Jia
• Format: Article
• Language: English
• Subjects: Acetates - pharmacology; Antioxidants - metabolism; Arachis - metabolism; Cyclopentanes - pharmacology; Germination; Methyl jasmonate; NaCl; Oxylipins - pharmacology; Peanut; Resveratrol; Resveratrol - pharmacology; Sodium Chloride - metabolism; Sodium Chloride - pharmacology
• ISSN: 0981-9428; 1873-2690
• DOI: 10.1016/j.plaphy.2022.12.012

In this study, the effects of methyl jasmonate (MeJA) and sodium chloride (NaCl) treatments on the resveratrol biosynthesis and physiology of peanuts during germination were investigated. The results showed that MeJA (150 μM) and NaCl (150 mM) treatments significantly promoted resveratrol biosynthesis in germinated peanuts. MeJA and NaCl treatments promoted resveratrol accumulation by regulating the activities of phenylalanine ammonia lyase (PAL), cinnamic acid 4-hydroxylase (C4H) and 4-coumarate coenzyme A ligase (4CL) and their gene expression levels in cotyledons and non-cotyledons. In addition, both MeJA and NaCl treatments inhibited peanut sprout growth, as evidenced by shorter sprout length, increased malondialdehyde content, and accumulation of reactive oxygen species in cotyledons and non-cotyledons. Both treatments' germinated peanuts responded to the environmental stimuli by raising the activities of antioxidant enzymes and controlling the levels of their gene' expression. Meanwhile, MeJA and NaCl treatments promoted Ca2+ aggregation in the root tips. Therefore, it can be deduced that Ca2+ may help improve the plant's resistance to adversity. In conclusion, treatment with MeJA (150 μM) or NaCl (150 mM) during germination is an effective way to enrich the resveratrol content of peanuts. Germinated peanuts enhance adaptation to the external environment by promoting resveratrol biosynthesis and enhancing antioxidant systems. •150 μM MeJA and 150 mM NaCl increased resveratrol content during peanut germination.•MeJA and NaCl regulated activity and gene expression of resveratrol biosyntheses•Both MeJA and NaCl treatments inhibited the growth of peanut sprouts.•Germinated peanut against MeJA and NaCl stress by boosting antioxidant system.