GC/MS and LC/MS Phytochemical Analysis of Vigna unguiculata L. Walp Pod

• Published in: Chemistry & biodiversity 2023-02, Vol.20 (2), p.e202200048-n/a
• Main Authors: Mahavirsing Dinore, Jaysing, Shivaji Patil, Harshal, Farooqui, Samreen, Pradhan, Vidya, Farooqui, Mazahar
• Format: Article
• Language: English
• Subjects: Ampicillin; antimicrobial activity; Antimicrobial agents; antioxidant activity; Antioxidants - chemistry; Carotenoids; Chromatography; Chromatography, Liquid; Constituents; cowpea; Cowpeas; E coli; Escherichia coli; Esters; Fatty acids; Flavonoids; Gas chromatography; Gas Chromatography-Mass Spectrometry; GC/MS; LC/MS; Liquid chromatography; Mass Spectrometry; Mass spectroscopy; Metabolites; Methanol; Minimum inhibitory concentration; Oxidants; Oxidizing agents; Phenolic compounds; Phenols; Phytochemicals; Phytochemicals - chemistry; Plant Extracts - chemistry; Scavenging; Scientific imaging; Spectral analysis; Spectrum analysis; Vigna - chemistry; Vigna unguiculata
• ISSN: 1612-1872; 1612-1880
• DOI: 10.1002/cbdv.202200048

Vigna unguiculata (L. Walp) or Cowpea pod methanolic extracts phytochemical analysis, total phenolic content (TPC), and secondary metabolite profiling were determined using gas chromatography‐mass spectrometry (GC/MS) and liquid chromatography‐mass spectrometry (LC/MS) analysis. GC/MS analysis revealed twenty compounds in the extract, while LC/MS analysis identified twenty‐four compounds. GC/MS chromatogram analysis suggested the presence of opioid α‐N‐Normethadol a major constituent found in methanolic extract and fatty acid esters carotenoid is found second major constituent. LC/MS chromatogram and the mass spectral analysis demonstrated the presence of flavonoids, carotenoids, and alkaloids as major phytochemicals. We investigated the antibacterial, anti‐fungal, and anti‐oxidant activity of pod methanolic extract. The extract was found equally effective against E. coli, S. pyogenes, and P. aeruginosa with MIC 100 μg/mL similar to the standard Ampicillin (MIC 100 μg/mL). C. albicans were found to be most susceptible to Vign unguiculata pods methanolic extract with a MIC of 250 μg/mL. The pod extract showed significant DPPH scavenging activity (IC50=78.38±0.15) which suggests its antioxidant potential.