The effects of medical linear accelerator X-rays on human peripheral blood lymphocytes in the presence of glucosamine

Glucosamine is widely prescribed as a dietary supplement used to treat arthritis. In this study, the radioprotective ability of glucosamine was evaluated against radiation-induced genotoxicity and cytotoxicity in human peripheral blood lymphocytes. Blood samples were collected from five healthy male...

Full description

Saved in:
Bibliographic Details
Published in:Medical oncology (Northwood, London, England) London, England), 2022-12, Vol.40 (1), p.65-65, Article 65
Main Authors: Rezapoor, Saeed, Ahmadi, Amirhossein, Shahbazian, Hojatollah, Cheki, Mohsen
Format: Article
Language:English
Subjects:
Apoptosis
Cancer therapies
Cell division
Cytotoxicity
DNA Damage
Gamma Rays
Glucosamine - pharmacology
Hematology
Humans
Internal Medicine
Lymphocytes
Male
Medical research
Medicine
Medicine & Public Health
Oncology
Original Paper
Pathology
Radiation therapy
Radiation-Protective Agents - pharmacology
Reactive Oxygen Species
Solvents
X-Rays
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Glucosamine is widely prescribed as a dietary supplement used to treat arthritis. In this study, the radioprotective ability of glucosamine was evaluated against radiation-induced genotoxicity and cytotoxicity in human peripheral blood lymphocytes. Blood samples were collected from five healthy male donors and were divided into four groups. Isolated lymphocytes and blood samples were treated with 10 µM of glucosamine for 2 h before exposure to 2 Gy radiation. The radioprotective potential of glucosamine was assessed by micronucleus assay, reactive oxygen species (ROS) level analysis, and flow cytometry. Irradiation significantly increased the micronuclei frequency as compared to the control group. Contrary to that pretreatment with glucosamine before irradiation significantly reduced the frequency of micronuclei. Furthermore, pretreatment with glucosamine significantly prevented the percentage of apoptotic lymphocytes. Also, glucosamine pretreatment significantly reduced the production of ROS in irradiated lymphocytes. This study shows glucosamine to be a potent radioprotector against radiation that induces DNA damage and apoptosis in human lymphocytes. Several additional in vivo and in vitro studies are needed before glucosamine can be considered as a radioprotective candidate in patients undergoing radiation therapy.
ISSN:1559-131X
1357-0560
1559-131X
DOI:10.1007/s12032-022-01936-0