Gastroprotective effect methanol extract of Caesalpinia coriaria pods against indomethacin- and ethanol-induced gastric lesions in Wistar rats

• Published in: Journal of ethnopharmacology 2023-04, Vol.305, p.116057-116057, Article 116057
• Main Authors: Pineda-Peña, Elizabeth Arlen, Capistran-Amezcua, David, Reyes-Ramírez, Adelfo, Xolalpa-Molina, Santiago, Chávez-Piña, Aracely Evangelina, Figueroa, Mario, Navarrete, Andrés
• Format: Article
• Language: English
• Subjects: Animals; Anti-inflammatory; Anti-Inflammatory Agents - therapeutic use; Anti-Ulcer Agents - pharmacology; Anti-Ulcer Agents - therapeutic use; Antioxidant; Antioxidants - therapeutic use; Caesalpinia; Caesalpinia coriaria; Ethanol; Ethanol - therapeutic use; Gallic Acid - therapeutic use; Gastric lesion; Indomethacin; Magnoliopsida; Methanol - therapeutic use; Phytotherapy; Plant Extracts - adverse effects; Rats; Rats, Wistar; Stomach Ulcer - chemically induced; Stomach Ulcer - drug therapy; Stomach Ulcer - prevention & control; Superoxide Dismutase; Tumor Necrosis Factor-alpha
• ISSN: 0378-8741; 1872-7573
• DOI: 10.1016/j.jep.2022.116057

Caesalpinia coriaria (Jacq.) Willd is widely used as a traditional medinal plant in Mexico for protective and healing purposes and the treatment of gastrointestinal diseases. To investigate the gastroprotective effect of extract of Caesalpinia coriaria pods against ethanol-induced and indomethacin-induced gastric lesion models, its anti-inflammatory and antioxidative activities, and its main compounds through LC-MS analysis. Male Wistar rats were orally administered a methanol extract obtained from the pods of C. coriaria at doses of 10, 30, 100, and 300 mg/kg prior to inducing gastric lesions with ethanol or indomethacin. Gastric mucosal lesions were evaluated by macroscopic and histopathological alterations. Determination of prostaglandin E2 (PGE2), alpha tumor necrosis factor (TNF-α), leukotriene B4 (LTB4), nitrites/nitrates, superoxide dismutase (SOD), and H2S gastric levels were investigated. Its main compounds of the active extract through LC-MS analysis. Phenolic compounds were identified as major components of methanol extract. LC-MS analysis identified 15 constituents, and the significant compounds were gallic acid, 3-O-galloylquinic acid, digalloylglucose, tetragalloylglucose, valoneic acid dilactone, pentagalloylglucose, digalloylshikimic acid, and ellagic acid. Pretreatment with the extract at doses of 100 and 300 mg/kg significantly reduced gastric ulcer lesions in both models. Compared with the reference drugs (omeprazole or ranitidine, respectively), no significant difference was found (p