Metabolic engineering of Aspergillus niger to enhance production of ethanol

This work describes the genetic transformation of a strain of Aspergillus niger with five different constructs containing 16 different heterologous genes, coding for four oxidoreductases, two cellobiohydrolases, one endoglucanase, one β‐glucosidase, six enzymes involved in xylose metabolism, and two...

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Published in:Biotechnology and applied biochemistry 2023-06, Vol.70 (3), p.1176-1188
Main Authors: los Santos Mondragón, Ara Itzel, Barragan, Brisia Alejandra Aguilar, Sánchez, Uriel Rojas, Calleja, Cristian Alberto López, Millán‐Chiu, Blanca E., Loske, Achim M., Lim, Miguel Angel Gómez
Format: Article
Language:English
Subjects:
Aspergillus niger
bioethanol
Bioprocessing
Carboxymethyl cellulose
Carboxymethylcellulose
Cellulose
consolidated bioprocessing
Enzymes
Ethanol
Fermentation
Genes
Genetic transformation
Glucosidase
lignocellulose
Metabolic engineering
Metabolism
Polymerase chain reaction
Substrates
Xylan
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Summary:This work describes the genetic transformation of a strain of Aspergillus niger with five different constructs containing 16 different heterologous genes, coding for four oxidoreductases, two cellobiohydrolases, one endoglucanase, one β‐glucosidase, six enzymes involved in xylose metabolism, and two enzymes involved in fermentation. The aim was to try and engineer a consolidated bioprocessing in A. niger. The fungus already contains most of these enzymes and we only enhanced endogenous activities. We recovered nine transformants containing all genes, as indicated by polymerase chain reaction (PCR). To confirm that the products of the genes were functional, we measured the activity of five different enzymes in all the strains, and they all showed enhanced activity over the wild‐type (wt) strain. The strains were grown on carboxymethyl cellulose (CMC) and xylan as substrates, and they produced considerably more ethanol than the wt. The levels of ethanol production were comparable to those reported in the literature.
ISSN:0885-4513
1470-8744
DOI:10.1002/bab.2430