Heparin-mimicking polymer-based hydrogel matrix regulates macrophage polarization by controlling cell adhesion

The physicochemical properties of biomaterials influence cell adhesion, shape, and polarization of macrophages. In this study, we aimed to evaluate the polarization of macrophages in terms of the regulation of cell adhesion and how synthetic mimics for heparin and poly(sodium-4-styrenesulfonate) can...

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Published in:Biochemical and biophysical research communications 2023-01, Vol.642, p.154-161
Main Authors: Jeong, Ji Hoon, Hur, Sung Sik, Lobionda, Stefani, Chaycham, Saharach, Oh, Jae Sang, Lee, Yun Kyung, Hwang, Yongsung
Format: Article
Language:English
Subjects:
Biomimetic Materials
Cell Adhesion
Cell spreading
Heparin - metabolism
Heparin - pharmacology
Hydrogel
Hydrogels
M1 macrophage
Macrophages - metabolism
Polarization
Polymers - metabolism
Polymers - pharmacology
Traction force microscopy
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Summary:The physicochemical properties of biomaterials influence cell adhesion, shape, and polarization of macrophages. In this study, we aimed to evaluate the polarization of macrophages in terms of the regulation of cell adhesion and how synthetic mimics for heparin and poly(sodium-4-styrenesulfonate) can regulate macrophage polarization by modulating cell shape, focal adhesion, cell traction force, and intracellular tension. Our initial findings showed that macrophages cultured with heparin-mimicking polymer-based hydrogel matrix showed reduced expression of cell adhesion markers such as integrins, vinculin, RhoA, and ROCK1/2 and reduced cell shape, elongation, cell-matrix traction force, and intracellular tension. Furthermore, we observed a significant decrease in cell adhesion in cells cultured on the hydrogel, resulting in the promotion of M1 polarization. These findings offer insights into the important roles of cell-matrix interactions in macrophage polarization and offer a platform for heparin-mimicking polymer-based hydrogel matrices to induce M1 polarization by inducing cell adhesion without classical activators. •M1 polarization is promoted by a heparin-mimicking polymer-based hydrogel matrix.•M1 macrophages polarized by LPS and heparin-mimicking polymer-based hydrogel matrix show reduced cell adhesion.•Heparin-mimicking polymer-based hydrogel matrix reduces cell adhesion and alters cell shape.•Heparin-mimicking polymer-based hydrogel matrix reduces traction force and intracellular tension of macrophages.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2022.12.049