Development of QuEChERS clean-up based on EMR-lipid for simultaneous analysis of 9 mycotoxins, acaylamide and 5-Hydroxymethylfurfural in biscuit by UHPLC-MS/MS

• Published in: Food chemistry 2023-05, Vol.409, p.135265-135265, Article 135265
• Main Authors: Long, You, Huang, Yousheng, Zhu, Mengting, Ma, Yingjie, Gan, Bei, Wang, YuanXing, Yu, Qiang, Xie, Jianhua, Chen, Yi
• Format: Article
• Language: English
• Subjects: 5-Hydroxymethylfurfural; Acrylamide; Chromatography, High Pressure Liquid - methods; EMR-lipid; Food analysis; Lipids; Mycotoxins; Mycotoxins - analysis; QuEChERS; Tandem Mass Spectrometry - methods; Thermally processed food
• ISSN: 0308-8146; 1873-7072
• DOI: 10.1016/j.foodchem.2022.135265

•Simultaneous detection method of 9 mycotoxins, AA and 5-HMF were established.•The QuEChERs extraction method with d-SPE with EMR-lipid was optimized.•All the LQQs of the method sacrificed the limit of EU regulation.•The recovery ranged from 72.11 to 115.59% and precision were lower than 15. A robust and sensitive analytical method for the simultaneous determination of 9 mycotoxins, AA, and 5-HMF by UHPLC-MS/MS was developed. Clean-up of the extracts was achieved by d-SPE with EMR-lipid. A new column phase (C18-PFP) was selected for HPLC separation after comparison with the C18 column. Finally, the method gave good linear relations with regression coefficients R2 > 0.99. The recovery of all the tested compounds was within the range of 70.67 to 104.88%, and the intraday and interday relative standard deviations (RSD) were lower than 12.49. The proposed method was then applied to investigate the mycotoxins, AA and 5-HMF in 20 food samples sold in the retail market. AA and 5-HMF were widely detected, and half of the samples were found to contain at least one mycotoxin contamination. Therefore, this method is potential to be used as a convenient and effective method for the cookies product quality control in the future.