Pinus massoniana needle extracts attenuate oxidative stress injury in cerebral ischemia reperfusion rats by regulating JNK3/caspase-3 signal transduction

• Published in: Zhejiang da xue xue bao. Journal of Zhejiang University. Medical sciences. Yi xue ban 2022-11, Vol.51 (5), p.563-572
• Main Authors: Zhang, Lei, Wang, Lingfeng, Hu, Peihao, Zhong, Xiaoming, Zhang, Danni, Qiu, Fengmei, Huang, Zhen
• Format: Article
• Language: English
• Subjects: Animals; Apoptosis; bcl-2-Associated X Protein - metabolism; bcl-2-Associated X Protein - pharmacology; bcl-2-Associated X Protein - therapeutic use; Brain Ischemia - drug therapy; Caspase 3 - metabolism; Caspase 3 - pharmacology; Cytochromes c - metabolism; Cytochromes c - pharmacology; Cytochromes c - therapeutic use; Edaravone - pharmacology; Edaravone - therapeutic use; Infarction, Middle Cerebral Artery; Male; Oxidative Stress; Pinus - chemistry; Plant Extracts - pharmacology; Rats; Rats, Sprague-Dawley; Reperfusion; Reperfusion Injury - drug therapy; Reperfusion Injury - prevention & control; Signal Transduction; Superoxide Dismutase
• ISSN: 1008-9292
• DOI: 10.3724/zdxbyxb-2022-0326

To investigate the effect and mechanism of needle extracts (PNE) on oxidative stress injury in cerebral ischemia reperfusion rats. The SD male rats were randomly divided into sham group, model control group, Edaravone (3 mg/kg) group, PNE low-dose (200 mg/kg), medium-dose (400 mg/kg) and high-dose (800 mg/kg) groups. PNE was administered by gavage for 7 d before modeling and 6 h after modeling in PNE treatment groups; Edaravone was given by intraperitoneal injection 7 d before modeling and 6 h after reperfusion. The rat model of cerebral ischemia reperfusion injury was established by middle cerebral artery occlusion method. After 24 h of reperfusion, the neurological deficit score, brain water content and cerebral infarction volume of rats were measured. The pathological changes of cerebral cortex and hippocampus were observed by HE staining, and the number of normal nerve cells was counted. The apoptosis rate of neurons in cerebral cortex was detected by TUNEL method. The content of nitric oxide (NO), malondialdehyde (MDA) and superoxide dismutase (SOD) activity in ischemic brain tissue were detected. The protein expression of c-Jun N-terminal kinase (JNK) 3, phosphorylated JNK3 (p-JNK3), B-cell lymphoma protein(Bcl) -2, Bcl-2 associated X (Bax), cytochrome C and caspase-3 in cerebral cortex were detected by Western blotting method. Compared with the model control group, the behavioral score, brain water content and cerebral infarction volume in PNE groups were significantly reduced (all