MOTS-c and aerobic exercise induce cardiac physiological adaptation via NRG1/ErbB4/CEBPβ modification in rats

• Published in: Life sciences (1973) 2023-02, Vol.315, p.121330-121330, Article 121330
• Main Authors: Yuan, Jinghan, Xu, Bowen, Ma, Jiacheng, Pang, Xiaoli, Fu, Yu, Liang, Min, Wang, Manda, Pan, Yanrong, Duan, Yimei, Tang, Mi, Zhu, Bingmei, Laher, Ismail, Li, Shunchang
• Format: Article
• Language: English
• Subjects: Aerobic exercise; Animals; C/EBPβ; Cardiac physiological adaptation; ErbB4; Exercise; MOTS-c; Myocardium - metabolism; Neuregulin 1; Neuregulin-1 - metabolism; Rats; Receptor, ErbB-4 - genetics; Receptor, ErbB-4 - metabolism; RNA, Messenger - metabolism
• ISSN: 0024-3205; 1879-0631
• DOI: 10.1016/j.lfs.2022.121330

To determine the effects of the mitochondrial open reading frame of the 12S ribosomal RNA type-c (MOTS-c) and aerobic exercise on cardiac structure and function and explore the role of neuregulin-1 (NRG1)- ErbB2 receptor tyrosine kinase 4(ErbB4)- CCAAT-enhancer binding protein β (C/EBPβ) in cardiac physiological adaptation induced by MOTS-c and aerobic training. We used Hematoxylin-Eosin staining(HE)and Transmission Electron Microscope (TEM) to observe the cardiac myocardial structure, carotid artery catheterization to test cardiac function, and real-time quantitative polymerase chain reaction (qRT-PCR) and Western blotting to describe the changes of NRG1, ErbB4, C/EBPβ, and Gata in cardiac physiological adaptation. MOTS-c and aerobic training significantly increased heart weight and heart weight index (HWI) (all p