Integrated transcriptomic and metabolomic analysis sheds new light on adaptation of Pinctada fucata martensii to short-term hypoxic stress

• Published in: Marine pollution bulletin 2023-02, Vol.187, p.114534-114534, Article 114534
• Main Authors: Chen, Jiayi, Qiu, Jinyu, Yang, Chuangye, Liao, Yongshan, He, Maoxiao, Mkuye, Robert, Li, Junhui, Deng, Yuewen, Du, Xiaodong
• Format: Article
• Language: English
• Subjects: Animals; Gene Expression Profiling; Hypoxic stress; Metabolome; Metabolomics; Pinctada - genetics; Pinctada fucata martensii; Transcriptome; Transcriptomics
• ISSN: 0025-326X; 1879-3363
• DOI: 10.1016/j.marpolbul.2022.114534

Analyses of the transcriptome and metabolome were conducted to clarify alterations of key genes and metabolites in pearl oysters following exposure to short-term hypoxic treatment. We totally detected 209 DEGs between the control and hypoxia groups. Enrichment analysis indicated the enrichment of GO terms including “oxidation-reduction process”, “ECM organization”, “chaperone cofactor-dependent protein refolding”, and “ECM-receptor interaction” KEGG pathway by the DEGs. In addition, between the two groups, a total of 28 SDMs were identified, which were implicated in 13 metabolic pathways, such as “phenylalanine metabolism”, “D-amino acid metabolism”, and “aminoacyl-tRNA biosynthesis”. Results suggest that pearl oysters are exposed to oxidative stress and apoptosis under short-term hypoxia. Also, pearl oysters might adapt to short-term hypoxic treatment by increasing antioxidant activity, modulating immune and biomineralization activities, maintaining protein homeostasis, and reorganizing the cytoskeleton. The results of our study help unveil the mechanisms by which pearl oysters respond adaptively to short-term hypoxia. •Pearl oysters are exposed to oxidative stress and apoptosis under short-term hypoxia.•Pearl oysters might adapt to short-term hypoxic treatment by increasing antioxidant activity.•Pearl oysters might adapt to short-term hypoxic stress by modulating immune activities.•Pearl oysters might adapt to short-term hypoxic treatment by maintaining protein homeostasis.•Pearl oysters might adapt to short-term hypoxic treatment by reorganizing the cytoskeleton.