Insights into the loss of glucoraphanin in post-harvested broccoli––Possible involvement of the declined supply capacity of sulfur donor

The loss of characteristic nutrient glucoraphanin during the shelf life seriously affects the nutritional quality of broccoli. Here, we monitored the changes in the levels of sulfur donors (cysteine and glutathione) required for glucoraphanin biosynthesis. Similar to glucoraphanin, cysteine content...

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Bibliographic Details
Published in:Plant science (Limerick) 2023-03, Vol.328, p.111580-111580, Article 111580
Main Authors: Yang, Qingxi, Luo, Manli, Zhou, Qian, Zhao, Yingbo, Chen, Jianye, Ji, Shujuan
Format: Article
Language:English
Subjects:
Brassica - genetics
Brassica - metabolism
Broccoli
Catabolism
Cysteine - metabolism
Glucoraphanin
Glucosinolates - metabolism
Glutathione - metabolism
Sulfur - metabolism
Sulfur donor
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Summary:The loss of characteristic nutrient glucoraphanin during the shelf life seriously affects the nutritional quality of broccoli. Here, we monitored the changes in the levels of sulfur donors (cysteine and glutathione) required for glucoraphanin biosynthesis. Similar to glucoraphanin, cysteine content decreased sharply. Continuous down-regulation of BoCysK1 and BoCysK2 genes encoding cysteine synthase might account for cysteine loss. Contrarily, glutathione content accumulated steadily, which might owe to the up-regulation of biosynthetic gene (BoEC1). Additionally, the change of malondialdehyde content was positively correlated with glutathione, implying that oxidative stress might stimulate glutathione accumulation. Nevertheless, the expression of BoGSTF11 gene encoding glutathione S-transferases was down-regulated, which blocked the supply of glutathione. The increase in the content of raphanusamic acid (degradation product) indicated that insufficient supply of sulfur donors not only could constrain the biosynthesis of glucoraphanin but also triggered its degradation. •The sharp reduction in cysteine content is responsible for the loss of glucoraphanin.•Cysteine level decreases due to the down-regulation of BoCysK1 and BoCysK2 genes.•Oxidative stress may stimulate glutathione accumulation in harvested broccoli.•Down-regulated expression of BoGSTF11 blocks glutathione supply to glucoraphanin.•Insufficient supply of sulfur donors may induce the decomposition of glucoraphanin.
ISSN:0168-9452
1873-2259
DOI:10.1016/j.plantsci.2022.111580