Unravelling structural, functional, evolutionary and genetic basis of SWEET transporters regulating abiotic stress tolerance in maize

• Published in: International journal of biological macromolecules 2023-02, Vol.229, p.539-560
• Main Authors: Vinodh Kumar, P.N., Mallikarjuna, Mallana Gowdra, Jha, Shailendra Kumar, Mahato, Anima, Lal, Shambhu Krishan, K.R., Yathish, Lohithaswa, Hirenallur Chandappa, Chinnusamy, Viswanathan
• Format: Article
• Language: English
• Subjects: Abiotic stress; Dominance; Evolution; Gene Expression Regulation, Plant; Genomics; Glucose; Maize; Membrane Transport Proteins - genetics; Molecular docking; Phylogeny; Plant Proteins - chemistry; Stress, Physiological - genetics; Sugars; SWEET transporters; Zea mays - genetics; Zea mays - metabolism
• ISSN: 0141-8130; 1879-0003
• DOI: 10.1016/j.ijbiomac.2022.12.326

Sugars Will Eventually be Exported Transporters (SWEETs) are the novel sugar transporters widely distributed among living systems. SWEETs play a crucial role in various bio-physiological processes, viz., plant developmental, nectar secretion, pollen development, and regulation of biotic and abiotic stresses, in addition to their prime sugar-transporting activity. Thus, in-depth structural, evolutionary, and functional characterization of maize SWEET transporters was performed for their utility in maize improvement. The mining of SWEET genes in the latest maize genome release (v.5) showed an uneven distribution of 20 ZmSWEETs. The comprehensive structural analyses and docking of ZmSWEETs with four sugars, viz., fructose, galactose, glucose, and sucrose, revealed frequent amino acid residues forming hydrogen (asparagine, valine, serine) and hydrophobic (tryptophan, glycine, and phenylalanine) interactions. Evolutionary analyses of SWEETs showed a mixed lineage with 50–100 % commonality of ortho-groups and -sequences evolved under strong purifying selection (Ka/Ks