Serodiagnosis of leishmaniasis in asymptomatic and symptomatic dogs by use of the recombinant dynamin-1-like protein from Leishmania infantum: A preliminary study

• Published in: Acta tropica 2023-03, Vol.239, p.106827-106827, Article 106827
• Main Authors: Siqueira, Williane Fernanda, Cardoso, Mariana Santos, Clímaco, Marianna de Carvalho, Silva, Ana Luiza Teixeira, Heidt, Benjamin, Eersels, Kasper, van Grinsven, Bart, Bartholomeu, Daniella Castanheira, Bueno, Lilian Lacerda, Cleij, Thomas, Fujiwara, Ricardo Toshio
• Format: Article
• Language: English
• Subjects: Animals; Antibodies, Protozoan; Antigens, Protozoan - genetics; Asymptomatic dogs; Canine leishmaniasis; Dog Diseases - diagnosis; Dog Diseases - parasitology; Dogs; Dynamin I; Enzyme-Linked Immunosorbent Assay; Immunodiagnostic; L. infantum; Leishmania infantum - genetics; Leishmaniasis, Visceral - diagnosis; Leishmaniasis, Visceral - veterinary; Recombinant antigens; Sensitivity and Specificity; Serologic Tests - veterinary; Symptomatic dog
• ISSN: 0001-706X; 1873-6254
• DOI: 10.1016/j.actatropica.2023.106827

•Better performance was obtained compared to more commonly used antigens.•Dyn-1 showed excellent ability to identify asymptomatic dogs (100% sensitivity).•Overall, the ability of rDyn-1 to identify dogs with CanL was 97% (sensitivity).•rDyn-1-based ELISA assays could be advantageous over classic diagnostic tests. Visceral leishmaniasis (VL) is a fatal manifestation of an infection caused by intracellular protozoa of the Leishmania genus. In New World countries, VL is classified as a zoonotic disease with domestic dogs acting as its main reservoir. Asymptomatic dogs are as competent to transmit Leishmania to the vectors as symptomatic dogs, however current diagnostic tests are limited and present low sensitivity for this important group. The development of accurate tests is fundamental to the early diagnosis, treatment, and control of canine leishmaniasis. In this study, we investigated the use of a recombinant protein (dynamin-1-like protein, Dyn-1) from L. infantum, as a potential target antigen for leishmaniasis serodiagnosis in both symptomatic and asymptomatic dogs. The antigenic performance of the protein was evaluated by means of ELISA assays using sera from symptomatic (n = 25), asymptomatic (n = 34) and non-infected dogs (n = 36) using ELISA. In addition, sera from dogs experimentally infected with Trypanosoma cruzi (n = 49) and naturally infected with Babesia sp. (n = 8) were tested to evaluate possible cross-reactivity. A crude soluble antigen (CSA) of Leishmania was used as an antigen control and K39 and K26 were used as reference antigens because they are already widely used in commercial tests. rDyn-1-based assay showed the highest sensitivity (97%) compared to the antigens K39 (88%), K26 (86%) and crude extract (95%). The highest specificity among the tests was also obtained with the protein rDyn-1 (94%), compared with the other antigens K39 (81%), K26 (87%), and crude extract (77%). This study showed that the rDyn-1 ELISA assay was able to identify 100% of asymptomatic dogs, establishing its potential as a target for the diagnosis of canine leishmaniasis.