A New OATP‐Mediated Hepatobiliary‐Specific Mn(II)‐Based MRI Contrast Agent for Hepatocellular Carcinoma in Mice: A Comparison With Gd‐EOB‐DTPA

Background Growing concerns about the safety of gadolinium (Gd)‐based contrast agents have reinforced the need for the development of Gd‐free MRI contrast agents (CAs) that are effective in imaging liver tumors. Purpose To evaluate the ability of Mn‐BnO‐TyEDTA MRI CA to detect hepatocellular carcino...

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Published in:Journal of magnetic resonance imaging 2023-09, Vol.58 (3), p.926-933
Main Authors: Xue, Yuan, Xiao, Bin, Xia, Zhiyang, Dai, Lixiong, Xia, Qian, Zhong, Lei, Zhu, Chunrong, Zhu, Jiang
Format: Article
Language:English
Subjects:
Animal models
Chromium
Contrast agents
Contrast media
Evaluation
Field strength
Fluorescence
Gadolinium
hepatobiliary‐specific MRI contrast agents
Hepatocellular carcinoma
hepatocellular carcinoma (HCC)
Image enhancement
Immunofluorescence
Inductively coupled plasma
Lesions
Liver
Liver cancer
Magnetic resonance imaging
magnetic resonance imaging (MRI)
Manganese (II)
Mass spectrometry
Mass spectroscopy
Medical imaging
Metal concentrations
Organic anion transporting polypeptide
Psoas muscle
Staining
Statistical analysis
Statistical tests
Tumors
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Summary:Background Growing concerns about the safety of gadolinium (Gd)‐based contrast agents have reinforced the need for the development of Gd‐free MRI contrast agents (CAs) that are effective in imaging liver tumors. Purpose To evaluate the ability of Mn‐BnO‐TyEDTA MRI CA to detect hepatocellular carcinoma in a mouse model of implanted liver tumor. Study Type Prospective. Animal Model Thirteen orthotopically implanted liver tumor mice. Field Strength/Sequence 3.0 T/precontrast and postcontrast T1‐weighted fast spoiled gradient recalled echo and T2‐weighted fast recovery fast spin‐echo imaging with fat suppression. Assessment The relative enhancement ratio was calculated and statistically compared. Lesion detection in postcontrast images was analyzed by calculations of area under the curve (AUC, the increases in liver‐to‐tumor contrast‐to‐noise ratio [∆CNR] vs. time curve). Mn or Gd levels were measured in the liver and tumoral tissues by inductively coupled plasma‐mass spectrometry. Tumor specimens were stained with hematoxylin and eosin (H&E) and the expression of organic anion transfer peptide (OATP)1B1 was evaluated by immunofluorescence (IF) staining and mean fluorescence intensity (MFI) was calculated. Statistical Tests Unpaired t‐test and two‐tailed paired t‐test. P 
ISSN:1053-1807
1522-2586
DOI:10.1002/jmri.28590