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Shikonin triggers GSDME-mediated pyroptosis in tumours by regulating autophagy via the ROS–MAPK14/p38α axis
•Shikonin induced GSDME-dependant pyroptosis in tumour cells.•The increase in intracellular ROS by SK treatment induced protective autophagy.•Repression of autophagy enhanced SK-induced pyroptosis.•MAPK14/p38α, a ROS sensor, modulate GSDME-dependant pyroptosis. Shikonin (SK), a botanical drug extrac...
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Published in: | Phytomedicine (Stuttgart) 2023-01, Vol.109, p.154596-154596, Article 154596 |
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Main Authors: | , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | •Shikonin induced GSDME-dependant pyroptosis in tumour cells.•The increase in intracellular ROS by SK treatment induced protective autophagy.•Repression of autophagy enhanced SK-induced pyroptosis.•MAPK14/p38α, a ROS sensor, modulate GSDME-dependant pyroptosis.
Shikonin (SK), a botanical drug extracted from Lithospermum erythrorhizon, has been shown to inhibit tumour growth through apoptosis and necrosis. However, whether SK induces pyroptosis in cancer cells is still unknown.
This study aims to investigated the mechanisms of SK-induced pyroptosis in tumour cells and mice.
In vivo and in vitro methods were used in this study. Cell deaths were analysed by LDH and CCK-8 assay and western blotting. To investigated the signalling pathway of SK-induced pyroptosis, various genes expressions were supressed by shRNA or inhibitors. High-sensitivity mass spectrometry assay was used to identified potential factors that regulate GSDME-mediated pyroptosis. Finally, a mouse model was used to investigate the effect of SK administration on tumour growth in vivo.
The activation of BAX/caspase-3 signalling was essential for GSDME-mediated pyroptosis by SK. Mechanistically, the intracellular reactive oxygen species (ROS) generation induced by SK treatment initiated GSDME-dependant pyroptosis. SK stimulation induced protective autophagy in a ROS-dependant manner, and repressed autophagy significantly enhanced SK-induced pyroptosis. Moreover, MAPK14/p38α, a ROS sensor, modulated SK-induced autophagy and ultimately affected GSDME-dependant pyroptosis.
Here, for the first time we demonstrated that SK treatment induced GSDME-dependant pyroptosis in tumour cells. Our results demonstrated that SK initiates ROS signalling to drive pyroptosis in cancer cells.
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ISSN: | 0944-7113 1618-095X |
DOI: | 10.1016/j.phymed.2022.154596 |