First detection and complete genome sequence of a new tobamovirus naturally infecting Hibiscus rosa-sinensis in Hawaii

High-throughput sequencing was used to analyze Hibiscus rosa-sinensis (family Malvaceae) plants with virus-like symptoms in Hawaii. Bioinformatic and phylogenetic analysis revealed the presence of two tobamoviruses, hibiscus latent Fort Pierce virus (HLFPV) and a new tobamovirus with the proposed na...

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Bibliographic Details
Published in:Archives of virology 2023-02, Vol.168 (2), p.40-40, Article 40
Main Authors: Wang, Xupeng, Larrea-Sarmiento, Adriana, Olmedo-Velarde, Alejandro, Kong, Alexandra, Borth, Wayne, Suzuki, Jon Y., Wall, Marisa M., Melzer, Michael, Hu, John
Format: Article
Language:English
Subjects:
Agricultural research
Annotated Sequence Record
Biomedical and Life Sciences
Biomedicine
Flowers & plants
Genome, Viral
Genomes
Hawaii
Hibiscus
Hibiscus rosa-sinensis
Infectious Diseases
Inoculation
Medical Microbiology
Next-generation sequencing
Nucleotide sequence
Phylogenetics
Phylogeny
Plant viruses
Proteins
RNA polymerase
Rosa
Tobacco
Tobamovirus - genetics
Viral infections
Virology
Viruses
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Summary:High-throughput sequencing was used to analyze Hibiscus rosa-sinensis (family Malvaceae) plants with virus-like symptoms in Hawaii. Bioinformatic and phylogenetic analysis revealed the presence of two tobamoviruses, hibiscus latent Fort Pierce virus (HLFPV) and a new tobamovirus with the proposed name "hibiscus latent Hawaii virus" (HLHV). This is the first report of the complete sequence, genome organization, and phylogenetic characterization of a tobamovirus infecting hibiscus in Hawaii. RT-PCR with virus-specific primers and Sanger sequencing further confirmed the presence of these viruses. Inoculation experiments showed that HLFPV could be mechanically transmitted to Nicotiana benthamiana and N. tabacum , while HLHV could only be mechanically transmitted to N. benthamiana.
ISSN:0304-8608
1432-8798
DOI:10.1007/s00705-022-05634-0