Electro-acupuncture treatment ameliorates intestinal inflammatory injury in cerebral ischemia–reperfusion rats via regulating the balance of Treg / γδ T cells

• Published in: Brain research 2023-03, Vol.1803, p.148233-148233, Article 148233
• Main Authors: Wang, Ya-Ling, Meng, Ling-Ling, Chen, Yong-Lin, OuYang, Ling, Wu, Bu-Fan, Peng, Rou, Lu, Sheng-Feng, Liu, Qing, Zhang, Tao, Li, Xiao-Xiao, Jing, Xin-yue, Fu, Shu-Ping, Xu, Bin
• Format: Article
• Language: English
• Subjects: Acupuncture Therapy; Animals; Brain Ischemia - metabolism; Brain Ischemia - therapy; Cerebral ischemia–reperfusion; EA treatment; Electroacupuncture - methods; Infarction, Middle Cerebral Artery - metabolism; Inflammation - therapy; Intestinal barrier; Intestinal injury; Ischemic Stroke; Rats; Rats, Sprague-Dawley; Reperfusion; Reperfusion Injury - metabolism; Reperfusion Injury - therapy; Stroke; T-Lymphocytes, Regulatory - metabolism; Treg/ γδ T cells
• ISSN: 0006-8993; 1872-6240
• DOI: 10.1016/j.brainres.2023.148233

•Collectively, our study documented, for the first time, that EA ameliorated intestinal inflammatory injury by modulating the Treg/ γδ T cell polarization in rats with I/R injury. This may be one of the mechanisms of EA conferring neuroprotection to ischemic stroke(Fig. 6). These findings will help us understand and further elaborate on the molecular biology of EA against ischemic stroke from the brain-gut axis. Electro-acupuncture (EA) has an anti-inflammatory role in ischemic stroke, but whether the protective effect of EA involves the regulation of the intestine barrier and Treg/ γδ T cells is unclear. Cerebral ischemia–reperfusion (I/R) injury was induced by middle cerebral artery occlusion(MCAO) for 2 h followed by reperfusion for 24 h. The rats have treated with EA at the “Baihui” acupoint(GV20). Triphenyl tetrazolium chloride (TTC) staining and Longa neurologic score were performed to evaluate the outcomes after ischemic stroke. Inflammatory factor expression levels in the serum, ischemic hemisphere brain, and small intestine were detected by ELISA or RT-qPCR. Additionally, the morphology change of the small intestine was evaluated by analyzing villus height and smooth muscle thickness. Meanwhile, the expression of tight-junction proteins, including Zonula Occludens-1 (ZO-1), Occludin, and Claudin-1, were detected to evaluate the impact of EA on mucosal permeability in the small intestine. The percentages of regulatory T cells (Tregs) (CD45+CD4+Foxp3+) and γδ T cells (CD45+CD4-γδ T+) were measured to assess the effect of EA on intestinal T cells. EA decreased the brain infarction volume and intestine barrier injury in ischemic stroke rats. At the same time, it effectively suppressed the post-stroke inflammation in the brain and small intestine. More importantly, EA treatment increased the percentage of Tregs in the small intestine while reducing the rate of γδ T cells, and ultimately increased the ratio of Treg/ γδ T cells. These results demonstrated that EA ameliorated intestinal inflammation damage by regulating the Treg/ γδ T cell polarity shift and improving the intestine barrier integrity in rats with I/R injury. This may be one of the mechanisms underlying the anti-ischemic injury effects of acupuncture on stroke.