Determination of biogenic amines in chicken, beef, and mutton by dansyl chloride microwave derivatization in Malaysia

In this study, an improved dansyl‐chloride derivatization technique using a microwave synthesizer was used for the qualitative and quantitative analyses of biogenic amine in the fresh meat samples. The derivatization technique was optimized in terms of temperature, reaction time, and spinning speed....

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Bibliographic Details
Published in:Journal of food science 2023-02, Vol.88 (2), p.650-665
Main Authors: Yue, Chen Son, Lim, Ah Kee, Chia, Meow Lin, Wong, Pei Yin, Chin, Joey Siew Rey, Wong, Weng Hang
Format: Article
Language:English
Subjects:
Amines
Animals
Beef
biogenic amine
Biogenic amines
Biogenic Amines - analysis
Cattle
Chickens
Chlorides
Chromatography, High Pressure Liquid - methods
fresh meat
Health risks
Histamine
HPLC
Liquid chromatography
Malaysia
Meat
microwave synthesizer
Microwaves
Mutton
Putrescine
Qualitative analysis
Reaction time
Tryptamine
Tryptamines
Tyramine
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Summary:In this study, an improved dansyl‐chloride derivatization technique using a microwave synthesizer was used for the qualitative and quantitative analyses of biogenic amine in the fresh meat samples. The derivatization technique was optimized in terms of temperature, reaction time, and spinning speed. The derivatization method together with a validated reversed‐phase HPLC‐DAD method was used for the determination of biogenic amines in chicken, beef, and mutton sold in the wet market. The results of the analyses showed that tryptamine, putrescine, and histamine were generally detected in all the three types of meat. Higher levels of histamine were found in chicken and beef. However, low levels of histamine were observed in mutton. Tyramine was either detected low or moderate in all the three types of meat. The biogenic amines of the fresh meat sold in the wet market is generally higher than the reported values. The mechanisms of biogenic amines‐dansyl‐chloride formation were investigated and proposed. Practical Application The biogenic amine derivatization method was improved. The improved derivatization method can be potentially used for various food products beside meats for routine biogenic amine analyses due to its fast analysis time and simplicity. High levels of biogenic amines were generally found in the meat sold in the wet markets. However, proper handling of the raw meat can reduce the risk of infection.
ISSN:0022-1147
1750-3841
DOI:10.1111/1750-3841.16404