Transcriptome analysis and differential expression in Arabidopsis thaliana in response to rohitukine (a chromone alkaloid) treatment

• Published in: Functional & integrative genomics 2023-03, Vol.23 (1), p.35-35, Article 35
• Main Authors: Ahmed, Sajad, Chouhan, Rekha, Junaid, Alim, Jamwal, Vijay Lakshmi, Thakur, Jitendra, Mir, Bilal Ahmad, Gandhi, Sumit G.
• Format: Article
• Language: English
• Subjects: Alkaloids; Animal Genetics and Genomics; Antineoplastic drugs; Antitumor agents; Arabidopsis thaliana; Biochemistry; Bioinformatics; Biomedical and Life Sciences; Cancer; Cell Biology; Flavopiridol; Immunomodulation; Inflammation; Life Sciences; Microbial Genetics and Genomics; Original Article; Photosynthesis; Plant Genetics and Genomics; Seedlings; Transcription factors; Transcriptomes; Transcriptomics
• ISSN: 1438-793X; 1438-7948
• DOI: 10.1007/s10142-023-00961-0

Rohitukine is a chromone alkaloid and precursor of potent anticancer drugs flavopiridol, P-276–00, and 2,6-dichloro-styryl derivative (11d) (IIIM-290). The metabolite is reported to possess anticancer, anti-inflammatory, antiadipogenic, immunomodulatory, gastroprotective, anti-implantation, antidyslipidemic, anti-arthritic, and anti-fertility properties. However, the physiological role of rohitukine in plant system is yet to be explored. Here, we studied the effect of rohitukine isolated from Dysoxylum gotadhora on Arabidopsis thaliana. The A. thaliana plants grown on a medium fortified with different rohitukine concentrations showed a significant effect on the growth and development. The root growth of A. thaliana seedlings showed considerable inhibition when grown on medium containing 1.0 mM of rohitukine. Transcriptomic analysis indicated the expression of 895 and 932 genes in control and treated samples respectively at a cut-off of FPKM ≥ 1 and P -value