Histone deacetylase inhibitor butyrate inhibits the cellular immunity and increases the serum immunity of pearl oyster Pinctada fucata martensii

• Published in: Fish & shellfish immunology 2023-02, Vol.133, p.108529-108529, Article 108529
• Main Authors: Yang, Jingmiao, Yang, Shuai, Liao, Yongshan, Deng, Yuewen, Jiao, Yu
• Format: Article
• Language: English
• Subjects: Animals; Butyrates - metabolism; Butyrates - pharmacology; Histone acetylation; Histone Deacetylase Inhibitors - metabolism; Histone Deacetylase Inhibitors - pharmacology; Immune response; Immunity, Cellular; Immunity, Innate - genetics; Lipopolysaccharides - metabolism; Lipopolysaccharides - pharmacology; Pinctada; Pinctada fucata martensii
• ISSN: 1050-4648; 1095-9947
• DOI: 10.1016/j.fsi.2023.108529

Histone acetylation is a dynamic epigenetic modification and sensitive to the changes in extracellular environment. Butyrate, a histone deacetylase inhibitor, can inhibit the deacetylation process of histones. In this study, we found that the acetylation level of H3 was enhanced at 12 h after lipopolysaccharide (LPS) stimulation and increased at 6 h after combining treatment with LPS and butyrate in pearl oyster Pinctada fucata martensii. Transcriptome analysis indicated that butyrate counter-regulated 29.95%–36.35% of the genes repressed by LPS, and these genes were mainly enriched in the “cell proliferation” and “Notch signaling pathway”. Meanwhile, butyrate inhibited the up-regulation of 31.54%–54.96% of the genes induced by LPS, and these genes were mainly enriched in “Notch signaling pathway”, “cell proliferation”, “NF-kappa B signaling pathway”, “TNF signaling pathway”, “apoptosis”, “NOD-like receptor signaling pathway”, “RIG-I-like receptor signaling pathway” and “cytosolic DNA-sensing pathway”. Gene expression analysis showed that butyrate downregulated most of cell proliferation, immune-related genes effected by LPS. The activities of LAP, LYS, ACP, ALP, and GSH-Px were up-regulated at 6 h after combining treatment with LPS and butyrate, suggesting that butyrate could activate serum immune-related enzymes in pearl oyster. These results can improve our understanding of the function of histone deacetylase in the immune response of pearl oyster and provide references for an in-depth study of the functions of histone deacetylase in mollusks. [Display omitted] •The acetylation level of H3 was enhanced after LPS and butyrate stimulation in pearl oyster.•Butyrate counter-regulated 29.95%–36.35% of the genes repressed by LPS.•Butyrate inhibited 31.54%–54.96% of the genes induced by LPS.•Butyrate downregulated most of cell cycle, immune-related genes induced by LPS.•Butyrate activated the serum immune-related enzymes in pearl oyster.