Activated neutrophils inhibit chemotactic migration of activated T lymphocytes to CXCL11 by multiple mechanisms

•Activated neutrophils strongly inhibits chemotaxis of activated T cells.•H2O2 released by PMA-stimulated neutrophils suppresses motility of activated T cells.•LPS-stimulated neutrophils inhibit chemotaxis of activated T cells via NETs formation.•Neutrophil elastase on NETs potently degrade CXCL11....

Full description

Saved in:
Bibliographic Details
Published in:Cellular immunology 2023-02, Vol.384, p.104663-104663, Article 104663
Main Authors: Tamura, Kohei, Miyato, Hideyo, Kanamaru, Rihito, Sadatomo, Ai, Takahashi, Kazuya, Ohzawa, Hideyuki, Koyanagi, Takahiro, Saga, Yasushi, Takei, Yuji, Fujiwara, Hiroyuki, Lefor, Alan Kawarai, Sata, Naohiro, Kitayama, Joji
Format: Article
Language:English
Subjects:
Chemokine CXCL11 - metabolism
Chemokines - metabolism
Chemotactic migration
CXCL11
Extracellular Traps - metabolism
Humans
Hydrogen Peroxide - metabolism
Lipopolysaccharides - metabolism
Lipopolysaccharides - pharmacology
Neutrophil extracellular traps
Neutrophils
T cells
T-Lymphocytes
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:•Activated neutrophils strongly inhibits chemotaxis of activated T cells.•H2O2 released by PMA-stimulated neutrophils suppresses motility of activated T cells.•LPS-stimulated neutrophils inhibit chemotaxis of activated T cells via NETs formation.•Neutrophil elastase on NETs potently degrade CXCL11. Accumulation of T lymphocytes and neutrophils shows inversed association with the prognosis of cancer patients, suggesting infiltration of neutrophils and T cells might be differently regulated in tumor tissue. In this study, we stimulated neutrophils with PMA or LPS to produce neutrophil extracellular traps (NETs) and examined the effects on chemotactic migration of activated T cells to a representative T cell chemokine, CXCL11. Migration of the activated T cells was totally abrogated by PMA-stimulated neutrophils placed either in upper or lower chamber, which was mostly canceled by pretreatment with Catalase. Although LPS-stimulated neutrophils also inhibited T cell migration, depletion of NETs by ultracentrifugation or degradation of NETs with DNAse I restored T cell migration. Western blots showed that LPS-stimulated neutrophils thoroughly degraded CXCL11 with NETs dependent manner. Activated neutrophils inhibit T cell chemotaxis via multiple mechanisms including the release of H2O2 and chemokine degradation by NETs, which may suppress adaptive immunity.
ISSN:0008-8749
1090-2163
DOI:10.1016/j.cellimm.2023.104663