Digital microfluidics for biological analysis and applications

Digital microfluidics (DMF) is an emerging liquid-handling technology based on arrays of microelectrodes for the precise manipulation of discrete droplets. DMF offers the benefits of automation, addressability, integration and dynamic configuration ability, and provides enclosed picoliter-to-microli...

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Bibliographic Details
Published in:Lab on a chip 2023-03, Vol.23 (5), p.1169-1191
Main Authors: Xu, Xing, Cai, Linfeng, Liang, Shanshan, Zhang, Qiannan, Lin, Shiyan, Li, Mingying, Yang, Qizheng, Li, Chong, Han, Ziyan, Yang, Chaoyong
Format: Article
Language:English
Subjects:
Actuation
Droplets
Electrowetting
Mechanical Phenomena
Microelectrodes
Microfluidic Analytical Techniques
Microfluidics
Nucleic Acids
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Summary:Digital microfluidics (DMF) is an emerging liquid-handling technology based on arrays of microelectrodes for the precise manipulation of discrete droplets. DMF offers the benefits of automation, addressability, integration and dynamic configuration ability, and provides enclosed picoliter-to-microliter reaction space, making it suitable for lab-on-a-chip biological analysis and applications that require high integration and intricate processes. A review of DMF bioassays with a special emphasis on those actuated by electrowetting on dielectric (EWOD) force is presented here. Firstly, a brief introduction is presented on both the theory of EWOD actuation and the types of droplet motion. Subsequently, a comprehensive overview of DMF-based biological analysis and applications, including nucleic acid, protein, immunoreaction and cell assays, is provided. Finally, a discussion on the strengths, challenges, and potential applications and perspectives in this field is presented.
ISSN:1473-0197
1473-0189
DOI:10.1039/d2lc00756h