Injectable platelet-rich fibrin positively regulates osteogenic differentiation of stem cells from implant hole via the ERK1/2 pathway

• Published in: Platelets (Edinburgh) 2023-12, Vol.34 (1), p.2159020-2159020
• Main Authors: Wang, Jia, Li, Wanxin, He, Xuxia, Li, Simei, Pan, Hongwei, Yin, Lihua
• Format: Article
• Language: English
• Subjects: Cell Differentiation; Cells, Cultured; ERK1/2; hBMSCs; Humans; injectable platelet-rich fibrin; MAP Kinase Signaling System; Osteogenesis; osteogenic differentiation; Platelet-Rich Fibrin; Stem Cells
• ISSN: 0953-7104; 1369-1635
• DOI: 10.1080/09537104.2022.2159020

Bone regeneration in dentistry is a dynamic approach for treating critical size bone defects that are unlikely to self-heal. Human bone marrow stem cell (hBMSCs) therapies are being tested clinically for various disorders and have remarkable clinical advancements in bone regeneration. Injectable platelet-rich fibrin (i-PRF), which is obtained from autologous blood centrifuged at 700 rpm (60 G) for 3 min can promote osteogenic differentiation of this cell, but the mechanism remains unclear. The objectives of this study were to explore the contents of i-PRF further and investigate its effect on the cell behavior of hBMSCs and the underlying molecular mechanisms. The results showed that i-PRF contained 41 cytokines, including macrophage colony-stimulating factor (M-CSF) and β-nerve growth factor (β-NGF), which had not been reported before. The Cell Counting Kit-8 and wound healing assay showed that 10% and 20% i-PRF improved the proliferation rate and the migration capacity of hBMSCs without toxicity to cells. Besides, the expression of osteogenic markers and the capacity to form mineralized nodules of hBMSCs were promoted by 20% i-PRF. Furthermore, i-PRF activated the ERK pathway, and the ERK inhibitor attenuated its effects. In summary, i-PRF promotes hBMSCs proliferation and migration and facilitates cell osteogenesis through the ERK pathway, which has promising potential in bone regeneration. What is the context? Bone defects caused by trauma or tumor is a great challenge in clinical practice. However, there is the good news that the bone defect in the oral can self-regenerate, the bone remodeling may take several months to several years and shows apparent individual differences. Different strategies, surgical techniques, and materials have been employed to induce an optimal outcome in guided bone regeneration. Blood products have been widely used in dentistry due to their excellent biocompatibility, growth factor content, ease of collection, and ability to be produced by the human body. Limited data suggest that Injectable platelet-rich fibrin positively regulates osteogenic differentiation of stem cells, but further evidence is needed to quantify this effect. What is new? It is unclear how many growth factors i-PRF contains in previous studies, so we detected 41 kinds of growth factors, more than has been previously appreciated, and found that all growth factors were measured in the samples, and the difference was in the amount of expression. In our res