Lipopolysaccharide induced neuroprotective effects of bacterial protease against Alzheimer's disease in male Wistar albino rats

• Published in: International journal of biological macromolecules 2023-03, Vol.230, p.123260-123260, Article 123260
• Main Authors: Azzam, Shaimaa M., Abdel Rahman, Amina A.S., Ahmed-Farid, Omar A., Abu El-Wafa, Wael Mohamed, Salem, Gad Elsayed Mohamed
• Format: Article
• Language: English
• Subjects: Acetylcholinesterase - metabolism; Alzheimer Disease - metabolism; Alzheimer's disease (AD); Animals; Bacillus cereus; Disease Models, Animal; Donepezil; Endopeptidases - metabolism; Hippocampus - metabolism; Lipopolysaccharides (LPS); Lipopolysaccharides - pharmacology; Male; Maze Learning; Memory Disorders - drug therapy; Neuroprotective Agents - therapeutic use; p-Tau protein; Peptide Hydrolases - metabolism; Protease; Rats; Rats, Wistar
• ISSN: 0141-8130; 1879-0003
• DOI: 10.1016/j.ijbiomac.2023.123260

Alzheimer's disease (AD) is a highly severe neurodegenerative condition that affects the hippocampus and is characterized by memory loss and dementia. This investigation aims to determine the potential of a bacterial protease enzyme produced by a new mutant strain of bacteria (Bacillus cereus S6-3/UM90) to influence the rat behavioural, biochemical, histological, and immuno-histochemical functions induced by lipopolysaccharides (LPS) experimentally. The administration of LPS exhibited a decline in memory performance via Morris' Water Maze test along with an elevation of IL-6, IL-17, amino acid neurotransmitters, Adenosine monophosphate (AMP), and 8-OHdG, whereas a decrease in ATP (Adenosine Triphosphate), monoamine transmitters, AChE (acetylcholinesterase) and PC (phosphatidylcholine). Additionally, there was a notable increase in GFAP (glial fibrillary acidic protein) and p-Tau protein immuno-expression levels along with obvious histological lesions in the hippocampal CA3 region. Moreover, the administration of protease or Donepezil restored the measured parameters to nearly normal levels and improved the histological architecture of the hippocampus and ameliorated memory impairments. In conclusion, the study provides evidence that the treatment with Bacterial protease can improve the memory and learning impairments of LPS–induced AD and may be used as a promising therapeutic agent to manage AD since it has anti-inflammatory and antioxidant effects. •Lipopolysaccharide (lPS) induce AD in rat model•LPS has toxic effect on brain tissue and increase tau protein and GFAP in brain.•Bacterial protease can alleviate the symptoms of AD.•Protease has potent anti-oxidant and anti-inflammatory.•Protease can maintain the brain tissue intact without any damage.