Detection of typical indigenous gut bacteria related to turmeric (Curcuma longa) powder in mouse caecum and human faecal cultures

• Published in: Molecular biology reports 2023-04, Vol.50 (4), p.2963-2974
• Main Authors: Xia, Yumeng, Lee, Gayang, Takei, Natsumi, Takahashi, Hajime, Kuda, Takashi
• Format: Article
• Language: English
• Subjects: Animal Anatomy; Animal Biochemistry; Animals; Antioxidants; Bacteria; Bacteria - genetics; Biomedical and Life Sciences; Cancer research; Cecum - microbiology; Curcuma; Curcuma longa; Fatty Acids, Volatile; Feces; Feces - microbiology; Gastrointestinal Microbiome - genetics; Histology; Humans; Inflammation; Intestinal microflora; Life Sciences; Mice; Microbiota; Morphology; Original Article; Powders; RNA, Ribosomal, 16S - genetics; rRNA 16S
• ISSN: 0301-4851; 1573-4978
• DOI: 10.1007/s11033-022-08237-9

Background Turmeric ( Curcuma longa ; TM) is widely used as a spice and possesses anti-inflammatory, antioxidant, and antibacterial properties. The relationship between TM functions and gut microbiota is still unclear. Methods and results To investigate the effect of TM on gut microbiota and to identify indigenous gut bacteria that are responsive to TM, we fed Institute of Cancer Research mice a diet containing either no fibre (NF, n  = 6) or 5% (w/w) TM ( n  = 6) for 14 days. Moreover, we obtained human stool samples from four healthy volunteers and incubated the samples without (control) or with 2% (w/v) TM at 37 °C for 24 h. Subsequently, microbiota analysis in murine caecal samples and human faecal cultures was performed using 16S rRNA (V4) amplicon sequencing. Higher faecal weights ( p