Solvent-Responsive Magnetic Beads for Accurate Detection of SARS-CoV‑2

Although numerous approaches were proposed for the nucleic acid (NA)-based SARS-CoV-2 detection, the nonideal NA desorption efficiency of conventional magnetic beads (MBs) limits their widespread application. In this study, we developed solvent-responsive MBs (called responsive MBs), which, in the p...

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Bibliographic Details
Published in:ACS applied materials & interfaces 2023-02, Vol.15 (4), p.4924-4934
Main Authors: Fei, Zhongjie, Liu, Ping, Cheng, Chu, Wei, Rongbin, Xiao, Pengfeng, Zhang, Youfa
Format: Article
Language:English
Subjects:
Biological and Medical Applications of Materials and Interfaces
Clinical Laboratory Techniques
COVID-19 - diagnosis
COVID-19 Testing
DNA
Humans
Magnetic Phenomena
Nucleic Acid Amplification Techniques
Real-Time Polymerase Chain Reaction
RNA, Viral - analysis
RNA, Viral - genetics
SARS-CoV-2
Sensitivity and Specificity
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Summary:Although numerous approaches were proposed for the nucleic acid (NA)-based SARS-CoV-2 detection, the nonideal NA desorption efficiency of conventional magnetic beads (MBs) limits their widespread application. In this study, we developed solvent-responsive MBs (called responsive MBs), which, in the presence of buffers, modulated the absorption and desorption capacities of NA by flipping the surface −COO–. Relative to other commercial MBs, responsive MBs exhibited similar absorption profiles and markedly enhanced desorption profiles. When applied for NA detection of complex samples, responsive MBs exhibited better performance of RNA detection than DNA, with obvious advantages in sensitivity. Specifically, the RNA and DNA desorption rates of commercial MBs were ∼85 and 82.5%, while those of responsive MBs were nearly 94 and 93.5%, respectively. Furthermore, responsive MBs exhibited remarkable extraction ability in a wide range of tissues and better performance of RNA extraction than DNA. When applied for SARS-CoV-2 detection, the responsive MBs along with the simulated digital RT-LAMP (a previously established apparatus) further improved detection efficiency, yielding a precise quantitative detection as low as 25 copies and an ultimate sensibility detection of 5 copies/mL. It was also successfully employed in numerous NA-based technologies such as polymerase chain reaction (PCR), sequencing, and so on.
ISSN:1944-8244
1944-8252
DOI:10.1021/acsami.2c18684