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Analysis of PAT1 subfamily members in the GRAS family of upland cotton and functional characterization of GhSCL13-2A in Verticillium dahliae resistance
Key message GhSCL13-2A, a member of the PAT1 subfamily in the GRAS family, positively regulates cotton resistance to Verticillium dahliae by mediating the jasmonic acid and salicylic acid signaling pathways and accumulation of reactive oxygen species. Verticillium wilt (VW) is a devastating disease...
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Published in: | Plant cell reports 2023-03, Vol.42 (3), p.487-504 |
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Main Authors: | , , , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | Key message
GhSCL13-2A, a member of the PAT1 subfamily in the GRAS family, positively regulates cotton resistance to
Verticillium dahliae
by mediating the jasmonic acid and salicylic acid signaling pathways and accumulation of reactive oxygen species.
Verticillium
wilt (VW) is a devastating disease of upland cotton (
Gossypium hirsutum
) that is primarily caused by the soil-borne fungus
Verticillium dahliae
. Scarecrow-like (SCL) proteins are known to be involved in plant abiotic and biotic stress responses, but their roles in cotton defense responses are still unclear. In this study, a total of 25 GhPAT1 subfamily members in the GRAS family were identified in upland cotton. Gene organization and protein domain analysis showed that GhPAT1 members were highly conserved.
GhPAT1
genes were widely expressed in various tissues and at multiple developmental stages, and they were responsive to jasmonic acid (JA), salicylic acid (SA), and ethylene (ET) signals. Furthermore,
GhSCL13-2A
was induced by
V. dahliae
infection.
V. dahliae
resistance was enhanced in
Arabidopsis thaliana
by ectopic overexpression of
GhSCL13-2A
, whereas cotton
GhSCL13-2A
knockdowns showed increased susceptibility. Levels of reactive oxygen species (ROS) and JA were also increased and SA content was decreased in
GhSCL13-2A
knockdowns. At the gene expression level,
PR
genes and SA signaling marker genes were down-regulated and JA signaling marker genes were upregulated in
GhSCL13-2A
knockdowns. GhSCL13-2A was shown to be localized to the cell membrane and the nucleus. Yeast two-hybrid and luciferase complementation assays indicated that GhSCL13-2A interacted with GhERF5. In
Arabidopsis
,
V. dahliae
resistance was enhanced by
GhERF5
overexpression; in cotton, resistance was reduced in
GhERF5
knockdowns. This study revealed a positive role of
GhSCL13-2A
in
V. dahliae
resistance, establishing it as a strong candidate gene for future breeding of
V. dahliae
-resistant cotton cultivars. |
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ISSN: | 0721-7714 1432-203X |
DOI: | 10.1007/s00299-022-02971-x |