Cytochrome-c-oxidase and ATPsynthase content increases in the endometrium of the patients with adenomyosis

Background Adenomyosis is characterized by overgrowth of endometrial glands and stroma in the myometrium and is associated with reduced apoptosis. One of the key participants in one of the pathways of apoptosis is cytochrome c , whose expression can be regulated by actin-binding proteins involved in...

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Bibliographic Details
Published in:Molecular biology reports 2023-04, Vol.50 (4), p.3919-3925
Main Authors: Toniyan, Konstantin A., Gorbacheva, Elena Yu, Golubkova, Maria A., Povorova, Victoria V., Boyarintsev, Valery V., Ogneva, Irina V.
Format: Article
Language:English
Subjects:
Actin
Adenomyosis - diagnosis
Adenomyosis - genetics
Adenomyosis - metabolism
Adenosine Triphosphate
Animal Anatomy
Animal Biochemistry
Apoptosis
ATP synthase
Biomedical and Life Sciences
Biopsy
Cytochrome
Cytochrome c
Cytochromes c - metabolism
Electron transport
Electron Transport Complex IV - genetics
Endometriosis - metabolism
Endometrium
Endometrium - metabolism
Female
Histology
Humans
Life Sciences
Microfilament Proteins - metabolism
Mitochondria
Morphology
mRNA
Myometrium
Protein biosynthesis
Proteins
RNA, Messenger - genetics
RNA, Messenger - metabolism
Short Communication
Stroma
Western blotting
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Summary:Background Adenomyosis is characterized by overgrowth of endometrial glands and stroma in the myometrium and is associated with reduced apoptosis. One of the key participants in one of the pathways of apoptosis is cytochrome c , whose expression can be regulated by actin-binding proteins involved in the formation of structures that provide cell motility. The aim of the study was to determine the content of actin-binding proteins, cytochrome c , and terminal members of the mitochondrial respiratory chain in endometrial biopsies of patients with adenomyosis and the control group. Methods and results The content of all studied proteins was determined by Western blotting, and the mRNA content of the corresponding genes was determined by quantitative RT-PCR. The relative content of alpha-actinin1 and mRNA of the gene encoding it in biopsy specimens from patients with adenomyosis was higher than in controls by 86 and 84% (p 
ISSN:0301-4851
1573-4978
DOI:10.1007/s11033-023-08269-9