CircRNA mannosidase alpha class 1A member 2 promotes esophageal squamous cell carcinoma progression by regulating C–C chemokine ligand 5

• Published in: Biochemical and biophysical research communications 2023-02, Vol.645, p.61-70
• Main Authors: Liu, Lie, Sang, Meixiang, Shi, Jian, Zheng, Yang, Meng, Lingjiao, Gu, Lina, Li, Ziyi, Liu, Fei, Bu, Jing, Duan, Xiaoyang, Zhao, Feifei, Zhang, Wentian, Shan, Baoen
• Format: Article
• Language: English
• Subjects: CCL5; Cell Line, Tumor; Cell Movement - genetics; Cell Proliferation - genetics; circMAN1A2; Circular RNA; Esophageal Neoplasms - pathology; Esophageal squamous cell carcinoma; Esophageal Squamous Cell Carcinoma - pathology; Gene Expression Regulation, Neoplastic; Humans; Ligands; Mannosidases - metabolism; MicroRNAs - genetics; RNA, Circular - genetics; RNA, Circular - metabolism
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/j.bbrc.2023.01.043

Esophageal squamous cell carcinoma (ESCC) is a common malignancy with high morbidity and mortality. Although circular RNAs (circRNAs) play important roles in various cancers including ESCC, the role of the circRNA mannosidase alpha class 1A member 2 (circMAN1A2) in ESCC has been rarely studied. This study aimed to explore the role of circMAN1A2 in ESCC. CircMAN1A2 expression in ESCC tissues and cells was evaluated, and the relationship between circMAN1A2 expression and prognosis in patients with ESCC was analyzed. C–C chemokine ligand 5 (CCL5) was found to be a downstream target of circMAN1A2 by analysing the Agilent Microarray. Next, we performed in vitro and in vivo xenotransplantation assays to explore the role of circMAN1A2 in ESCC. We observed that high circMAN1A2 expression is associated with poor prognosis in patients with ESCC. Suppression of circMAN1A2 expression inhibits the proliferation, migration, and invasiveness of ESCC via regulating CCL5. Our results suggest that circMAN1A2 can promote the progression of ESCC by regulating CCL5. Thus, circMAN1A2 might be a novel diagnostic biomarker of ESCC, and targeting circMAN1A2 using inhibitors could be a potential therapeutic strategy to treat ESCC. [Display omitted] •CircMANA2 is highly expressed in ESCC tissues and cells.•Down-regulation of circMAN1A2 inhibits the proliferation, migration and invasiveness of ESCC.•CircMAN1A2 may be a novel diagnostic biomarker and therapeutic target of ESCC.