Association between genetic variants in key vitamin‐D‐pathway genes and external apical root resorption linked to orthodontic treatment

• Published in: European journal of oral sciences 2023-04, Vol.131 (2), p.e12916-n/a
• Main Authors: Marañón‐Vásquez, G., Küchler, EC, Hermann, S., Paddenberg, E, Schröder, A., Baratto‐Filho, F., Flores‐Mir, C., Proff, P., Kirschneck, C.
• Format: Article
• Language: English
• Subjects: 25-Hydroxyvitamin D3 1-alpha-Hydroxylase - genetics; 25‐hydroxyvitamin D3 1‐alpha‐hydroxylase; Alleles; Calciferol; Case-Control Studies; Cytochrome; Cytochrome P450; Cytochromes P450; Genes; Genetic diversity; Genetic Predisposition to Disease; Genetic variance; Genotype; Genotypes; Haplotypes; Homozygotes; Humans; Nucleotides; Orthodontics; Polymorphism, Single Nucleotide; receptors, calcitriol; Receptors, Calcitriol - genetics; Root resorption; Root Resorption - diagnostic imaging; Root Resorption - genetics; Single-nucleotide polymorphism; Tooth root; Vitamin D; Vitamin D receptors; vitamin D3 24‐hydroxylase; vitamin D‐binding protein; Vitamins
• ISSN: 0909-8836; 1600-0722
• DOI: 10.1111/eos.12916

This study evaluated the association between single‐nucleotide polymorphisms (SNPs) in vitamin‐D‐related genes and the amount of external apical root resorption linked to orthodontic treatment. One hundred and forty‐three individuals were assessed. The amount of external apical root resorption of upper central incisors (EARRinc) and lower first molars (EARRmol) were evaluated in radiographs. Seven SNPs were genotyped across four genes including the vitamin D receptor [VDR], group‐specific component [GC], cytochrome P450 family 27 subfamily B member 1 [CYP27B1], and cytochrome P450 family 24 subfamily A member 1 [CYP24A1]. Linear regressions were implemented to determine allele‐effects on external apical root resorption. Individuals carrying the AA genotype in VDR rs2228570 had a 21% higher EARRmol than those having AG and GG genotypes (95% CI: 1.03,1.40). EARRmol in heterozygous rs2228570, was 12% lower than for homozygotes (95%CI: 0.78,0.99). Participants with the CCG haplotype (rs1544410‐rs7975232‐rs731236) in VDR had an EARRmol 16% lower than those who did not carry this haplotype. Regarding CYP27B1 rs4646536, EARRinc in participants who had at least one G allele was 42% lower than for homozygotes AA (95%CI: 0.37,0.93). Although these results did not remain significant after multiple testing adjustment, potential associations may still be suggested. Further replication studies are needed to confirm or refute these findings.