Loading…
Recovery and Manipulation of Nanoparticulate Bioproducts: Relevance to the Up-Scaled Manufacture of Gene Therapy Vectors
Purified inclusion bodies (∼140 nm diameter) of yeast α-glucosidase sourced from recombinant Escherichia coli, and particles (∼120 nm diameter) fabricated from bovine serum albumin, have been identified as potential surrogate mimics of viral gene therapy vectors. Their ready availability enabled the...
Saved in:
Published in: | Food and bioproducts processing 2000-03, Vol.78 (1), p.11-18 |
---|---|
Main Authors: | , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
cited_by | cdi_FETCH-LOGICAL-c349t-b85a47b8b28657d8831640ee820c15e02d505e21255bcc30e56adf9348f794f43 |
---|---|
cites | cdi_FETCH-LOGICAL-c349t-b85a47b8b28657d8831640ee820c15e02d505e21255bcc30e56adf9348f794f43 |
container_end_page | 18 |
container_issue | 1 |
container_start_page | 11 |
container_title | Food and bioproducts processing |
container_volume | 78 |
creator | Braas, G.M.F. Walker, S.G. Zhang, Z. Lyddiatt, A. |
description | Purified inclusion bodies (∼140 nm diameter) of yeast α-glucosidase sourced from recombinant
Escherichia coli, and particles (∼120 nm diameter) fabricated from bovine serum albumin, have been identified as potential surrogate mimics of viral gene therapy vectors. Their ready availability enabled the collection of detailed process data relevant to the implementation of virus recovery in a manner not possible with
bona fide vectors. Partition of unwashed and washed inclusion body preparations, and purified albumin particles, in PEG-phosphate aqueous two-phase systems (ATPS) was compared with that for enveloped and non-enveloped viruses. System intensification achieved a volumetric capacity for nanoparticulates in ATPS which exceeded that for adsorbents commonly applied to fuidised bed recovery of protein products. This observation is discussed in the context of future productive needs in commercial gene therapy, and the current shortage of adsorbents custom-designed for nanoparticulate recovery. |
doi_str_mv | 10.1205/096030800532671 |
format | article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_27690152</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0960308500701854</els_id><sourcerecordid>27690152</sourcerecordid><originalsourceid>FETCH-LOGICAL-c349t-b85a47b8b28657d8831640ee820c15e02d505e21255bcc30e56adf9348f794f43</originalsourceid><addsrcrecordid>eNp1kMtLxDAQxoMouD7OXnMQb9VJmrSpNxVf4AN012vJplOM1KYm6eL-92ZdQRA8Dcx88818P0IOGBwzDvIEqgJyUAAy50XJNsiElUJkuSzZJpmsplkay22yE8IbADDF5IR8PqFxC_RLqvuG3uveDmOno3U9dS190L0btI_WrJpIz60bvGtGE8MpfcIOF7o3SKOj8RXpbMieje7w22dstYmjx5XNNfZIp6_o9bCkL2ii82GPbLW6C7j_U3fJ7OpyenGT3T1e316c3WUmF1XM5kpqUc7VnKtClo1SOSsEICoOhkkE3kiQyBmXcm5MDigL3bRVLlRbVqIV-S45Wvumxz9GDLF-t8Fg1-ke3RhqXhYVMMmT8GQtNN6F4LGtB2_ftV_WDOoV4foP4bRx-GOtQ8rd-gTDht81wRSviiSr1jJMMRcWfR2MxcStsT6xqBtn_z3xBWAXjQA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>27690152</pqid></control><display><type>article</type><title>Recovery and Manipulation of Nanoparticulate Bioproducts: Relevance to the Up-Scaled Manufacture of Gene Therapy Vectors</title><source>ScienceDirect Freedom Collection 2022-2024</source><creator>Braas, G.M.F. ; Walker, S.G. ; Zhang, Z. ; Lyddiatt, A.</creator><creatorcontrib>Braas, G.M.F. ; Walker, S.G. ; Zhang, Z. ; Lyddiatt, A.</creatorcontrib><description>Purified inclusion bodies (∼140 nm diameter) of yeast α-glucosidase sourced from recombinant
Escherichia coli, and particles (∼120 nm diameter) fabricated from bovine serum albumin, have been identified as potential surrogate mimics of viral gene therapy vectors. Their ready availability enabled the collection of detailed process data relevant to the implementation of virus recovery in a manner not possible with
bona fide vectors. Partition of unwashed and washed inclusion body preparations, and purified albumin particles, in PEG-phosphate aqueous two-phase systems (ATPS) was compared with that for enveloped and non-enveloped viruses. System intensification achieved a volumetric capacity for nanoparticulates in ATPS which exceeded that for adsorbents commonly applied to fuidised bed recovery of protein products. This observation is discussed in the context of future productive needs in commercial gene therapy, and the current shortage of adsorbents custom-designed for nanoparticulate recovery.</description><identifier>ISSN: 0960-3085</identifier><identifier>EISSN: 1744-3571</identifier><identifier>DOI: 10.1205/096030800532671</identifier><language>eng</language><publisher>Rugby: Elsevier B.V</publisher><subject>adsorption ; aqueous two phase systems ; Biological and medical sciences ; bioprocessing ; Biotechnology ; Fundamental and applied biological sciences. Psychology ; Gene therapy ; Health. Pharmaceutical industry ; Industrial applications and implications. Economical aspects ; nanoparticle processing ; protein particles ; viral mimics</subject><ispartof>Food and bioproducts processing, 2000-03, Vol.78 (1), p.11-18</ispartof><rights>2000 The Institution of Chemical Engineers</rights><rights>2000 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c349t-b85a47b8b28657d8831640ee820c15e02d505e21255bcc30e56adf9348f794f43</citedby><cites>FETCH-LOGICAL-c349t-b85a47b8b28657d8831640ee820c15e02d505e21255bcc30e56adf9348f794f43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>309,310,314,780,784,789,790,23930,23931,25140,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1418296$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Braas, G.M.F.</creatorcontrib><creatorcontrib>Walker, S.G.</creatorcontrib><creatorcontrib>Zhang, Z.</creatorcontrib><creatorcontrib>Lyddiatt, A.</creatorcontrib><title>Recovery and Manipulation of Nanoparticulate Bioproducts: Relevance to the Up-Scaled Manufacture of Gene Therapy Vectors</title><title>Food and bioproducts processing</title><description>Purified inclusion bodies (∼140 nm diameter) of yeast α-glucosidase sourced from recombinant
Escherichia coli, and particles (∼120 nm diameter) fabricated from bovine serum albumin, have been identified as potential surrogate mimics of viral gene therapy vectors. Their ready availability enabled the collection of detailed process data relevant to the implementation of virus recovery in a manner not possible with
bona fide vectors. Partition of unwashed and washed inclusion body preparations, and purified albumin particles, in PEG-phosphate aqueous two-phase systems (ATPS) was compared with that for enveloped and non-enveloped viruses. System intensification achieved a volumetric capacity for nanoparticulates in ATPS which exceeded that for adsorbents commonly applied to fuidised bed recovery of protein products. This observation is discussed in the context of future productive needs in commercial gene therapy, and the current shortage of adsorbents custom-designed for nanoparticulate recovery.</description><subject>adsorption</subject><subject>aqueous two phase systems</subject><subject>Biological and medical sciences</subject><subject>bioprocessing</subject><subject>Biotechnology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene therapy</subject><subject>Health. Pharmaceutical industry</subject><subject>Industrial applications and implications. Economical aspects</subject><subject>nanoparticle processing</subject><subject>protein particles</subject><subject>viral mimics</subject><issn>0960-3085</issn><issn>1744-3571</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><recordid>eNp1kMtLxDAQxoMouD7OXnMQb9VJmrSpNxVf4AN012vJplOM1KYm6eL-92ZdQRA8Dcx88818P0IOGBwzDvIEqgJyUAAy50XJNsiElUJkuSzZJpmsplkay22yE8IbADDF5IR8PqFxC_RLqvuG3uveDmOno3U9dS190L0btI_WrJpIz60bvGtGE8MpfcIOF7o3SKOj8RXpbMieje7w22dstYmjx5XNNfZIp6_o9bCkL2ii82GPbLW6C7j_U3fJ7OpyenGT3T1e316c3WUmF1XM5kpqUc7VnKtClo1SOSsEICoOhkkE3kiQyBmXcm5MDigL3bRVLlRbVqIV-S45Wvumxz9GDLF-t8Fg1-ke3RhqXhYVMMmT8GQtNN6F4LGtB2_ftV_WDOoV4foP4bRx-GOtQ8rd-gTDht81wRSviiSr1jJMMRcWfR2MxcStsT6xqBtn_z3xBWAXjQA</recordid><startdate>20000301</startdate><enddate>20000301</enddate><creator>Braas, G.M.F.</creator><creator>Walker, S.G.</creator><creator>Zhang, Z.</creator><creator>Lyddiatt, A.</creator><general>Elsevier B.V</general><general>Institution of Chemical Engineers</general><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>F28</scope><scope>FR3</scope></search><sort><creationdate>20000301</creationdate><title>Recovery and Manipulation of Nanoparticulate Bioproducts: Relevance to the Up-Scaled Manufacture of Gene Therapy Vectors</title><author>Braas, G.M.F. ; Walker, S.G. ; Zhang, Z. ; Lyddiatt, A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c349t-b85a47b8b28657d8831640ee820c15e02d505e21255bcc30e56adf9348f794f43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>adsorption</topic><topic>aqueous two phase systems</topic><topic>Biological and medical sciences</topic><topic>bioprocessing</topic><topic>Biotechnology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene therapy</topic><topic>Health. Pharmaceutical industry</topic><topic>Industrial applications and implications. Economical aspects</topic><topic>nanoparticle processing</topic><topic>protein particles</topic><topic>viral mimics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Braas, G.M.F.</creatorcontrib><creatorcontrib>Walker, S.G.</creatorcontrib><creatorcontrib>Zhang, Z.</creatorcontrib><creatorcontrib>Lyddiatt, A.</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><jtitle>Food and bioproducts processing</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Braas, G.M.F.</au><au>Walker, S.G.</au><au>Zhang, Z.</au><au>Lyddiatt, A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Recovery and Manipulation of Nanoparticulate Bioproducts: Relevance to the Up-Scaled Manufacture of Gene Therapy Vectors</atitle><jtitle>Food and bioproducts processing</jtitle><date>2000-03-01</date><risdate>2000</risdate><volume>78</volume><issue>1</issue><spage>11</spage><epage>18</epage><pages>11-18</pages><issn>0960-3085</issn><eissn>1744-3571</eissn><abstract>Purified inclusion bodies (∼140 nm diameter) of yeast α-glucosidase sourced from recombinant
Escherichia coli, and particles (∼120 nm diameter) fabricated from bovine serum albumin, have been identified as potential surrogate mimics of viral gene therapy vectors. Their ready availability enabled the collection of detailed process data relevant to the implementation of virus recovery in a manner not possible with
bona fide vectors. Partition of unwashed and washed inclusion body preparations, and purified albumin particles, in PEG-phosphate aqueous two-phase systems (ATPS) was compared with that for enveloped and non-enveloped viruses. System intensification achieved a volumetric capacity for nanoparticulates in ATPS which exceeded that for adsorbents commonly applied to fuidised bed recovery of protein products. This observation is discussed in the context of future productive needs in commercial gene therapy, and the current shortage of adsorbents custom-designed for nanoparticulate recovery.</abstract><cop>Rugby</cop><pub>Elsevier B.V</pub><doi>10.1205/096030800532671</doi><tpages>8</tpages></addata></record> |
fulltext | fulltext |
identifier | ISSN: 0960-3085 |
ispartof | Food and bioproducts processing, 2000-03, Vol.78 (1), p.11-18 |
issn | 0960-3085 1744-3571 |
language | eng |
recordid | cdi_proquest_miscellaneous_27690152 |
source | ScienceDirect Freedom Collection 2022-2024 |
subjects | adsorption aqueous two phase systems Biological and medical sciences bioprocessing Biotechnology Fundamental and applied biological sciences. Psychology Gene therapy Health. Pharmaceutical industry Industrial applications and implications. Economical aspects nanoparticle processing protein particles viral mimics |
title | Recovery and Manipulation of Nanoparticulate Bioproducts: Relevance to the Up-Scaled Manufacture of Gene Therapy Vectors |
url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T07%3A08%3A37IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Recovery%20and%20Manipulation%20of%20Nanoparticulate%20Bioproducts:%20Relevance%20to%20the%20Up-Scaled%20Manufacture%20of%20Gene%20Therapy%20Vectors&rft.jtitle=Food%20and%20bioproducts%20processing&rft.au=Braas,%20G.M.F.&rft.date=2000-03-01&rft.volume=78&rft.issue=1&rft.spage=11&rft.epage=18&rft.pages=11-18&rft.issn=0960-3085&rft.eissn=1744-3571&rft_id=info:doi/10.1205/096030800532671&rft_dat=%3Cproquest_cross%3E27690152%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c349t-b85a47b8b28657d8831640ee820c15e02d505e21255bcc30e56adf9348f794f43%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=27690152&rft_id=info:pmid/&rfr_iscdi=true |