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Syringic acid ameliorates ischemia/reperfusion-induced testicular injury in rats via suppressing of HMGB1/NF-κB axis and endoplasmic reticulum stress
Purpose To investigate the possible protective role of syringic acid on torsion/detorsion-induced testicular injury using biochemical and histopathological approaches for the first time. Methods A total of 24 rats were divided into 4 groups: sham control, torsion/detorsion, torsion/detorsion + syrin...
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| Published in: | European journal of trauma and emergency surgery (Munich : 2007) 2023-06, Vol.49 (3), p.1595-1602 |
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| container_issue | 3 |
| container_start_page | 1595 |
| container_title | European journal of trauma and emergency surgery (Munich : 2007) |
| container_volume | 49 |
| creator | Demir, Elif Ayazoglu Demir, Selim Kazaz, Ilke Onur Kucuk, Hatice Alemdar, Nihal Turkmen Gecici, Omer Faruk Mentese, Ahmet Aliyazicioglu, Yuksel |
| description | Purpose
To investigate the possible protective role of syringic acid on torsion/detorsion-induced testicular injury using biochemical and histopathological approaches for the first time.
Methods
A total of 24 rats were divided into 4 groups: sham control, torsion/detorsion, torsion/detorsion + syringic acid (50 mg/kg and 100 mg/kg). Tissue malondialdehyde, total oxidant status and total antioxidant status levels were determined using colorimetric methods. Tissue 8-hydroxy-2′-deoxyguanosine, superoxide dismutase, catalase, high mobility group box 1, nuclear factor kappa B protein 65, tumor necrosis factor-alpha, interleukin-6, myeloperoxidase, 78-kDa glucose-regulated protein, activating transcription factor-6, C/EBP homologous protein and caspase-3 levels were determined using commercial enzyme-linked immunosorbent assay kits. Johnsen’s testicle scoring system was used for histological evaluation.
Results
Compared with the control group, the levels of oxidative stress, inflammation, endoplasmic reticulum stress and apoptosis were significantly increased in the torsion/detorsion group (
p
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| doi_str_mv | 10.1007/s00068-023-02227-7 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2769373696</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2769373696</sourcerecordid><originalsourceid>FETCH-LOGICAL-c375t-145fda621512243b571a8b3442bea559adeba90d21a46938eead0431abd2ebc73</originalsourceid><addsrcrecordid>eNp9kUtuFDEQhi0EIiFwARbIEhs2zfjV7u4liUiCFGABrK3qdjV41C9cY8RcJIfJITgT7kwIEgsWVnnx_Z9d-hl7LsVrKUS1ISGErQuhdD5KVUX1gB3L2uqiaYx8eH_X-og9IdpmWthSPWZH2tpGlcIcs-tP-ximr6Hj0AXPYcQhzBF2SDxQ9w3HAJuIC8Y-UZinIkw-deh5BnahSwNEHqZtivs8eM4R_xGAU1qWiETZzOeeX76_OJWbD-fFr5tTDj8DcZg8x8nPywA05scj3trSyGm3Bp-yRz0MhM_u5gn7cv7289llcfXx4t3Zm6ui01W5K6Qpew9WyVIqZXRbVhLqVhujWoSybMBjC43wSoKxja4RwQujJbReYdtV-oS9OniXOH9PeSc35rVxGGDCOZFTVY5V2jY2oy__QbdzilP-nVO1ErU0Qq1CdaC6OBNF7N0Swwhx76Rwa2vu0JrLrbnb1twaenGnTu2I_j7yp6YM6ANAy9oWxr9v_0f7G9tOpaE</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2820814027</pqid></control><display><type>article</type><title>Syringic acid ameliorates ischemia/reperfusion-induced testicular injury in rats via suppressing of HMGB1/NF-κB axis and endoplasmic reticulum stress</title><source>Springer Nature</source><creator>Demir, Elif Ayazoglu ; Demir, Selim ; Kazaz, Ilke Onur ; Kucuk, Hatice ; Alemdar, Nihal Turkmen ; Gecici, Omer Faruk ; Mentese, Ahmet ; Aliyazicioglu, Yuksel</creator><creatorcontrib>Demir, Elif Ayazoglu ; Demir, Selim ; Kazaz, Ilke Onur ; Kucuk, Hatice ; Alemdar, Nihal Turkmen ; Gecici, Omer Faruk ; Mentese, Ahmet ; Aliyazicioglu, Yuksel</creatorcontrib><description>Purpose
To investigate the possible protective role of syringic acid on torsion/detorsion-induced testicular injury using biochemical and histopathological approaches for the first time.
Methods
A total of 24 rats were divided into 4 groups: sham control, torsion/detorsion, torsion/detorsion + syringic acid (50 mg/kg and 100 mg/kg). Tissue malondialdehyde, total oxidant status and total antioxidant status levels were determined using colorimetric methods. Tissue 8-hydroxy-2′-deoxyguanosine, superoxide dismutase, catalase, high mobility group box 1, nuclear factor kappa B protein 65, tumor necrosis factor-alpha, interleukin-6, myeloperoxidase, 78-kDa glucose-regulated protein, activating transcription factor-6, C/EBP homologous protein and caspase-3 levels were determined using commercial enzyme-linked immunosorbent assay kits. Johnsen’s testicle scoring system was used for histological evaluation.
Results
Compared with the control group, the levels of oxidative stress, inflammation, endoplasmic reticulum stress and apoptosis were significantly increased in the torsion/detorsion group (
p
< 0.05). Syringic acid administrations statistically significantly restored these damage in a dose dependent manner (
p
< 0.05). Moreover, it was found that the results of histological examinations supported the biochemical results to a statistically significant extent.
Conclusion
The overall results suggest that syringic acid emerges as a potential compound for the treatment of testicular torsion and may be subject to clinical trials.</description><identifier>ISSN: 1863-9933</identifier><identifier>EISSN: 1863-9941</identifier><identifier>DOI: 10.1007/s00068-023-02227-7</identifier><identifier>PMID: 36692504</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Acids ; Animals ; Apoptosis ; Critical Care Medicine ; Emergency medical care ; Emergency Medicine ; Endoplasmic reticulum ; Endoplasmic Reticulum Stress ; HMGB1 Protein - metabolism ; Intensive ; Ischemia ; Male ; Medicine ; Medicine & Public Health ; NF-kappa B - metabolism ; Original Article ; Oxidative stress ; Proteins ; Rats ; Reperfusion ; Sports Medicine ; Surgery ; Surgical Orthopedics ; Testis - metabolism ; Testis - pathology ; Traumatic Surgery</subject><ispartof>European journal of trauma and emergency surgery (Munich : 2007), 2023-06, Vol.49 (3), p.1595-1602</ispartof><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Germany 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c375t-145fda621512243b571a8b3442bea559adeba90d21a46938eead0431abd2ebc73</citedby><cites>FETCH-LOGICAL-c375t-145fda621512243b571a8b3442bea559adeba90d21a46938eead0431abd2ebc73</cites><orcidid>0000-0002-1863-6280</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36692504$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Demir, Elif Ayazoglu</creatorcontrib><creatorcontrib>Demir, Selim</creatorcontrib><creatorcontrib>Kazaz, Ilke Onur</creatorcontrib><creatorcontrib>Kucuk, Hatice</creatorcontrib><creatorcontrib>Alemdar, Nihal Turkmen</creatorcontrib><creatorcontrib>Gecici, Omer Faruk</creatorcontrib><creatorcontrib>Mentese, Ahmet</creatorcontrib><creatorcontrib>Aliyazicioglu, Yuksel</creatorcontrib><title>Syringic acid ameliorates ischemia/reperfusion-induced testicular injury in rats via suppressing of HMGB1/NF-κB axis and endoplasmic reticulum stress</title><title>European journal of trauma and emergency surgery (Munich : 2007)</title><addtitle>Eur J Trauma Emerg Surg</addtitle><addtitle>Eur J Trauma Emerg Surg</addtitle><description>Purpose
To investigate the possible protective role of syringic acid on torsion/detorsion-induced testicular injury using biochemical and histopathological approaches for the first time.
Methods
A total of 24 rats were divided into 4 groups: sham control, torsion/detorsion, torsion/detorsion + syringic acid (50 mg/kg and 100 mg/kg). Tissue malondialdehyde, total oxidant status and total antioxidant status levels were determined using colorimetric methods. Tissue 8-hydroxy-2′-deoxyguanosine, superoxide dismutase, catalase, high mobility group box 1, nuclear factor kappa B protein 65, tumor necrosis factor-alpha, interleukin-6, myeloperoxidase, 78-kDa glucose-regulated protein, activating transcription factor-6, C/EBP homologous protein and caspase-3 levels were determined using commercial enzyme-linked immunosorbent assay kits. Johnsen’s testicle scoring system was used for histological evaluation.
Results
Compared with the control group, the levels of oxidative stress, inflammation, endoplasmic reticulum stress and apoptosis were significantly increased in the torsion/detorsion group (
p
< 0.05). Syringic acid administrations statistically significantly restored these damage in a dose dependent manner (
p
< 0.05). Moreover, it was found that the results of histological examinations supported the biochemical results to a statistically significant extent.
Conclusion
The overall results suggest that syringic acid emerges as a potential compound for the treatment of testicular torsion and may be subject to clinical trials.</description><subject>Acids</subject><subject>Animals</subject><subject>Apoptosis</subject><subject>Critical Care Medicine</subject><subject>Emergency medical care</subject><subject>Emergency Medicine</subject><subject>Endoplasmic reticulum</subject><subject>Endoplasmic Reticulum Stress</subject><subject>HMGB1 Protein - metabolism</subject><subject>Intensive</subject><subject>Ischemia</subject><subject>Male</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>NF-kappa B - metabolism</subject><subject>Original Article</subject><subject>Oxidative stress</subject><subject>Proteins</subject><subject>Rats</subject><subject>Reperfusion</subject><subject>Sports Medicine</subject><subject>Surgery</subject><subject>Surgical Orthopedics</subject><subject>Testis - metabolism</subject><subject>Testis - pathology</subject><subject>Traumatic Surgery</subject><issn>1863-9933</issn><issn>1863-9941</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><recordid>eNp9kUtuFDEQhi0EIiFwARbIEhs2zfjV7u4liUiCFGABrK3qdjV41C9cY8RcJIfJITgT7kwIEgsWVnnx_Z9d-hl7LsVrKUS1ISGErQuhdD5KVUX1gB3L2uqiaYx8eH_X-og9IdpmWthSPWZH2tpGlcIcs-tP-ximr6Hj0AXPYcQhzBF2SDxQ9w3HAJuIC8Y-UZinIkw-deh5BnahSwNEHqZtivs8eM4R_xGAU1qWiETZzOeeX76_OJWbD-fFr5tTDj8DcZg8x8nPywA05scj3trSyGm3Bp-yRz0MhM_u5gn7cv7289llcfXx4t3Zm6ui01W5K6Qpew9WyVIqZXRbVhLqVhujWoSybMBjC43wSoKxja4RwQujJbReYdtV-oS9OniXOH9PeSc35rVxGGDCOZFTVY5V2jY2oy__QbdzilP-nVO1ErU0Qq1CdaC6OBNF7N0Swwhx76Rwa2vu0JrLrbnb1twaenGnTu2I_j7yp6YM6ANAy9oWxr9v_0f7G9tOpaE</recordid><startdate>20230601</startdate><enddate>20230601</enddate><creator>Demir, Elif Ayazoglu</creator><creator>Demir, Selim</creator><creator>Kazaz, Ilke Onur</creator><creator>Kucuk, Hatice</creator><creator>Alemdar, Nihal Turkmen</creator><creator>Gecici, Omer Faruk</creator><creator>Mentese, Ahmet</creator><creator>Aliyazicioglu, Yuksel</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>K9.</scope><scope>KB0</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>MBDVC</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-1863-6280</orcidid></search><sort><creationdate>20230601</creationdate><title>Syringic acid ameliorates ischemia/reperfusion-induced testicular injury in rats via suppressing of HMGB1/NF-κB axis and endoplasmic reticulum stress</title><author>Demir, Elif Ayazoglu ; Demir, Selim ; Kazaz, Ilke Onur ; Kucuk, Hatice ; Alemdar, Nihal Turkmen ; Gecici, Omer Faruk ; Mentese, Ahmet ; Aliyazicioglu, Yuksel</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c375t-145fda621512243b571a8b3442bea559adeba90d21a46938eead0431abd2ebc73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Acids</topic><topic>Animals</topic><topic>Apoptosis</topic><topic>Critical Care Medicine</topic><topic>Emergency medical care</topic><topic>Emergency Medicine</topic><topic>Endoplasmic reticulum</topic><topic>Endoplasmic Reticulum Stress</topic><topic>HMGB1 Protein - metabolism</topic><topic>Intensive</topic><topic>Ischemia</topic><topic>Male</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>NF-kappa B - metabolism</topic><topic>Original Article</topic><topic>Oxidative stress</topic><topic>Proteins</topic><topic>Rats</topic><topic>Reperfusion</topic><topic>Sports Medicine</topic><topic>Surgery</topic><topic>Surgical Orthopedics</topic><topic>Testis - metabolism</topic><topic>Testis - pathology</topic><topic>Traumatic Surgery</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Demir, Elif Ayazoglu</creatorcontrib><creatorcontrib>Demir, Selim</creatorcontrib><creatorcontrib>Kazaz, Ilke Onur</creatorcontrib><creatorcontrib>Kucuk, Hatice</creatorcontrib><creatorcontrib>Alemdar, Nihal Turkmen</creatorcontrib><creatorcontrib>Gecici, Omer Faruk</creatorcontrib><creatorcontrib>Mentese, Ahmet</creatorcontrib><creatorcontrib>Aliyazicioglu, Yuksel</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>ProQuest Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest Research Library</collection><collection>Research Library (Corporate)</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>European journal of trauma and emergency surgery (Munich : 2007)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Demir, Elif Ayazoglu</au><au>Demir, Selim</au><au>Kazaz, Ilke Onur</au><au>Kucuk, Hatice</au><au>Alemdar, Nihal Turkmen</au><au>Gecici, Omer Faruk</au><au>Mentese, Ahmet</au><au>Aliyazicioglu, Yuksel</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Syringic acid ameliorates ischemia/reperfusion-induced testicular injury in rats via suppressing of HMGB1/NF-κB axis and endoplasmic reticulum stress</atitle><jtitle>European journal of trauma and emergency surgery (Munich : 2007)</jtitle><stitle>Eur J Trauma Emerg Surg</stitle><addtitle>Eur J Trauma Emerg Surg</addtitle><date>2023-06-01</date><risdate>2023</risdate><volume>49</volume><issue>3</issue><spage>1595</spage><epage>1602</epage><pages>1595-1602</pages><issn>1863-9933</issn><eissn>1863-9941</eissn><abstract>Purpose
To investigate the possible protective role of syringic acid on torsion/detorsion-induced testicular injury using biochemical and histopathological approaches for the first time.
Methods
A total of 24 rats were divided into 4 groups: sham control, torsion/detorsion, torsion/detorsion + syringic acid (50 mg/kg and 100 mg/kg). Tissue malondialdehyde, total oxidant status and total antioxidant status levels were determined using colorimetric methods. Tissue 8-hydroxy-2′-deoxyguanosine, superoxide dismutase, catalase, high mobility group box 1, nuclear factor kappa B protein 65, tumor necrosis factor-alpha, interleukin-6, myeloperoxidase, 78-kDa glucose-regulated protein, activating transcription factor-6, C/EBP homologous protein and caspase-3 levels were determined using commercial enzyme-linked immunosorbent assay kits. Johnsen’s testicle scoring system was used for histological evaluation.
Results
Compared with the control group, the levels of oxidative stress, inflammation, endoplasmic reticulum stress and apoptosis were significantly increased in the torsion/detorsion group (
p
< 0.05). Syringic acid administrations statistically significantly restored these damage in a dose dependent manner (
p
< 0.05). Moreover, it was found that the results of histological examinations supported the biochemical results to a statistically significant extent.
Conclusion
The overall results suggest that syringic acid emerges as a potential compound for the treatment of testicular torsion and may be subject to clinical trials.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>36692504</pmid><doi>10.1007/s00068-023-02227-7</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0002-1863-6280</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1863-9933 |
| ispartof | European journal of trauma and emergency surgery (Munich : 2007), 2023-06, Vol.49 (3), p.1595-1602 |
| issn | 1863-9933 1863-9941 |
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| source | Springer Nature |
| subjects | Acids Animals Apoptosis Critical Care Medicine Emergency medical care Emergency Medicine Endoplasmic reticulum Endoplasmic Reticulum Stress HMGB1 Protein - metabolism Intensive Ischemia Male Medicine Medicine & Public Health NF-kappa B - metabolism Original Article Oxidative stress Proteins Rats Reperfusion Sports Medicine Surgery Surgical Orthopedics Testis - metabolism Testis - pathology Traumatic Surgery |
| title | Syringic acid ameliorates ischemia/reperfusion-induced testicular injury in rats via suppressing of HMGB1/NF-κB axis and endoplasmic reticulum stress |
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