Ectopic expression of MmCYP1A1, a mouse cytochrome P450 gene, positively regulates stress tolerance in apple calli and Arabidopsis

• Published in: Plant cell reports 2023-02, Vol.42 (2), p.433-448
• Main Authors: Wang, Xiao-Na, Yang, Fei, Zhang, Jiu-Cheng, Ren, Yi-Ran, An, Jian-Ping, Chang, Da-Yong, Wang, Xiao-Fei, You, Chun-Xiang
• Format: Article
• Language: English
• Subjects: Animals; Apples; Arabidopsis; Arabidopsis - genetics; Arabidopsis - metabolism; Biomedical and Life Sciences; Biotechnology; Cell Biology; Cytochrome; Cytochrome P-450 Enzyme System - genetics; Cytochrome P-450 Enzyme System - metabolism; Cytochrome P450; Cytochrome P450 monooxygenase; Cytochromes P450; Drought; Droughts; Ectopic expression; Ectopic Gene Expression; Electrolyte leakage; Fruits; Gene expression; Gene Expression Regulation, Plant; Hydrogen peroxide; Hydrogen Peroxide - metabolism; Kinases; Life Sciences; Malus - genetics; Malus - metabolism; Melatonin; Melatonin - metabolism; Mice; Mus musculus; Original Article; Osmotic stress; Plant Biochemistry; Plant Proteins - genetics; Plant Proteins - metabolism; Plant Sciences; Plant stress; Plants (botany); Plants, Genetically Modified - metabolism; Polyethylene glycol; Stomata; Stress, Physiological - genetics; Water loss
• ISSN: 0721-7714; 1432-203X
• DOI: 10.1007/s00299-022-02969-5

Key message Ectopic expression of MmCYP1A1 gene from Mus musculus in apple calli and Arabidopsis increased the levels of melatonin and 6-hydroxymelatonin, and improved their stress resistance. Melatonin occurs widely in organisms, playing a key regulatory role. CYP1A1 is a cytochrome P450 monooxygenase, involved in the melatonin metabolism, and is responsible for the synthesis of 6-hydroxymelatonin from melatonin. Melatonin and 6-hydroxymelatonin have strong antioxidant activities in animals. Here, we cloned MmCYP1A1 from Mus musculus and found that ectopic expression of MmCYP1A1 improved the levels of melatonin and 6-hydroxymelatonin in transgenic apple calli and Arabidopsis . Subsequently, we observed that MmCYP1A1 increased the tolerance of transgenic apple calli and Arabidopsis to osmotic stress simulated by polyethylene glycol 6000 (PEG 6000), as well as resistance of transgenic Arabidopsis to drought stress. Further, the number of lateral roots of MmCYP1A1 transgenic Arabidopsis were enhanced significantly after PEG 6000 treatment. The expression of MmCYP1A1 remarkably reduced malondialdehyde (MDA) content, electrolyte leakage, accumulation of H 2 O 2 and O 2 − during stress treatment. Moreover, MmCYP1A1 enhanced stress tolerance in apple calli and Arabidopsis by increasing the expression levels of resistance genes. MmCYP1A1 also promoted stomatal closure in transgenic Arabidopsis to reduce leaf water loss during drought. Our results indicate that MmCYP1A1 plays a key role in plant stress tolerance, which may provide a reference for future plant stress tolerance studies.