Bi-enzyme competition based on ZIF-67 co-immobilization for real-time monitoring of exocellular ATP

Monitoring exocellular adenosine-5′-triphosphate (ATP) is a demanding task but the biosensor development is limited by the low concentration and rapid degradation of ATP. Herein, we developed a simple yet effective biosensor based on ZIF-67 loaded with bi-enzymes of glucose (GOx) and hexokinase (HEX...

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Bibliographic Details
Published in:Mikrochimica acta (1966) 2023-02, Vol.190 (2), p.71-71, Article 71
Main Authors: Lu, Yan, Li, Junmin, Liu, Yuqiao, Zhu, Ling, Xiao, Shenghao, Bai, Mingxia, Chen, Dajing, Xie, Tian
Format: Article
Language:English
Subjects:
Adenosine
Adenosine Triphosphate - chemistry
Analytical Chemistry
Animals
Biosensors
Characterization and Evaluation of Materials
Chemistry
Chemistry and Materials Science
Cobalt
Confined spaces
Detectors
Dextrose
Enzymes
Enzymes, Immobilized - chemistry
Glucose
Glucose Oxidase - chemistry
Glycolysis
Hexokinase
Hexokinase - chemistry
Hexokinase - metabolism
Microengineering
Monitoring
Nanochemistry
Nanotechnology
Original Paper
Porous media
Rats
Real time
Silver
Substrates
Time measurement
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Summary:Monitoring exocellular adenosine-5′-triphosphate (ATP) is a demanding task but the biosensor development is limited by the low concentration and rapid degradation of ATP. Herein, we developed a simple yet effective biosensor based on ZIF-67 loaded with bi-enzymes of glucose (GOx) and hexokinase (HEX) for effective detection of ATP. In the confined space of the porous matrix, the bi-enzymes competed for the glucose substrate in the presence of ATP, facilitating the biosensor to detect low ATP concentrations down to the micromole level (3.75 μM) at working potential of 0.55 V (vs. Ag/AgCl). Furthermore, ZIF-67 with cobalt served as a porous matrix to specifically adsorb ATP molecules, allowing it to differentiate isomers with sensitivity of 0.53 nA/μM, RSD of 5.4%, and recovery rate of 93.3%. We successfully applied the fabricated biosensor to measure ATP secreted from rat PC12 cells in the pericellular space thus realizing time-resolving measurement. This work paved the path for real-time monitoring of ATP released by cells, which will aid in understanding tumor cell glycolysis and immune responses. Graphical abstract
ISSN:0026-3672
1436-5073
DOI:10.1007/s00604-023-05652-y