Fabrication and Characterization of Piezoelectric Polymer Composites and Cytocompatibility with Mesenchymal Stem Cells

• Published in: ACS applied materials & interfaces 2023-01, Vol.15 (3), p.3731-3743
• Main Authors: Sheng, Ruoyu, Mu, Jing, Chernozem, Roman V., Mukhortova, Yulia R., Surmeneva, Maria A., Pariy, Igor O., Ludwig, Tim, Mathur, Sanjay, Xu, Changlu, Surmenev, Roman A., Liu, Huinan Hannah
• Format: Article
• Language: English
• Subjects: Biological and Medical Applications of Materials and Interfaces; Graphite - chemistry; Graphite - pharmacology; Mesenchymal Stem Cells; Polymers - chemistry; Polymers - pharmacology
• ISSN: 1944-8244; 1944-8252
• DOI: 10.1021/acsami.2c15802

Piezoelectric materials are promising for biomedical applications because they can provide mechanical or electrical stimulations via converse or direct piezoelectric effects. The stimulations have been proven to be beneficial for cell proliferation and tissue regeneration. Recent reports showed that doping different contents of reduced graphene oxide (rGO) or polyaniline (PANi) into biodegradable polyhydroxybutyrate (PHB) enhanced their piezoelectric response, showing potential for biomedical applications. In this study, we aim to determine the correlation between physiochemical properties and the in vitro cell response to the PHB-based composite scaffolds with rGO or PANi. Specifically, we characterized the surface morphology, wetting behavior, electrochemical impedance, and piezoelectric properties of the composites and controls. The addition of rGO and PANi resulted in decreased fiber diameters and hydrophobicity of PHB. The increased surface energy of PHB after doping nanofillers led to a reduced water contact angle (WCA) from 101.84 ± 2.18° (for PHB) to 88.43 ± 0.83° after the addition of 3 wt % PANi, whereas doping 1 wt % rGO decreased the WCA value to 92.56 ± 2.43°. Meanwhile, doping 0.2 wt % rGO into PHB improved the piezoelectric properties compared to the PHB control and other composites. Adding up to 1 wt % rGO or 3 wt % PANi nanofillers in PHB did not affect the adhesion densities of bone marrow-derived mesenchymal stem cells (BMSCs) on the scaffolds. The aspect ratios of attached BMSCs on the composite scaffolds increased compared to the PHB control. The study indicated that the PHB-based composites are promising for potential applications such as regenerative medicine, tissue stimulation, and bio-sensing, which should be further studied.