Effect on hypoxia/reoxygenation-induced cardiomyocyte injury and Pink1/Parkin pathway

Our study aimed to detect the effects of proprotein convertase subtilisin/kexin type 9 (PCSK9) on exacerbating cardiomyocyte hypoxia/reoxygenation (H/R) injury and the possible mechanism. A cell model of H/R was constructed. PCSK9 mRNA and protein levels were significantly upregulated during AC16 ca...

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Published in:General physiology and biophysics 2023-01, Vol.42 (1), p.87-95
Main Authors: Lu, Xiyang, Huang, Guangwei, Bao, Hailong, Duan, Zonggang, Li, Chao, Lin, Muzhi, Zhou, Haiyan, Luo, Zhenhua, Li, Wei
Format: Article
Language:English
Subjects:
Apoptosis
Autophagy
Humans
Hypoxia - metabolism
Myocytes, Cardiac
Proprotein Convertase 9 - metabolism
Ubiquitin-Protein Ligases - genetics
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container_end_page 95
container_issue 1
container_start_page 87
container_title General physiology and biophysics
container_volume 42
creator Lu, Xiyang
Huang, Guangwei
Bao, Hailong
Duan, Zonggang
Li, Chao
Lin, Muzhi
Zhou, Haiyan
Luo, Zhenhua
Li, Wei
description Our study aimed to detect the effects of proprotein convertase subtilisin/kexin type 9 (PCSK9) on exacerbating cardiomyocyte hypoxia/reoxygenation (H/R) injury and the possible mechanism. A cell model of H/R was constructed. PCSK9 mRNA and protein levels were significantly upregulated during AC16 cardiomyocyte H/R. Flowmetry detection of apoptosis, as well as JC-1, confirmed that PCSK9 upregulation of autophagy levels was accompanied by apoptosis. Furthermore, in the H/R+si-PCSK9 group, the expression of autophagy-related protein LC3 decreased and P62 increased. At the same time, the presentation of the autophagic pathway Pink1/Parkin was also downregulated. In conclusion, in AC16 cardiomyocytes treated with H/R, PCSK9 expression and autophagy levels were increased; a possible molecular mechanism was the activation of the Pink1/Parkin pathway.
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subjects Apoptosis
Autophagy
Humans
Hypoxia - metabolism
Myocytes, Cardiac
Proprotein Convertase 9 - metabolism
Ubiquitin-Protein Ligases - genetics
title Effect on hypoxia/reoxygenation-induced cardiomyocyte injury and Pink1/Parkin pathway
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