CRISPR‐Cas Biochemistry and CRISPR‐Based Molecular Diagnostics
Polymerase chain reaction (PCR)‐based nucleic acid testing has played a critical role in disease diagnostics, pathogen surveillance, and many more. However, this method requires a long turnaround time, expensive equipment, and trained personnel, limiting its widespread availability and diagnostic ca...
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Published in: | Angewandte Chemie International Edition 2023-04, Vol.62 (17), p.e202214987-n/a |
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Main Authors: | , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | Polymerase chain reaction (PCR)‐based nucleic acid testing has played a critical role in disease diagnostics, pathogen surveillance, and many more. However, this method requires a long turnaround time, expensive equipment, and trained personnel, limiting its widespread availability and diagnostic capacity. On the other hand, the clustered regularly interspaced short palindromic repeats (CRISPR) technology has recently demonstrated capability for nucleic acid detection with high sensitivity and specificity. CRISPR‐mediated biosensing holds great promise for revolutionizing nucleic acid testing procedures and developing point‐of‐care diagnostics. This review focuses on recent developments in both fundamental CRISPR biochemistry and CRISPR‐based nucleic acid detection techniques. Four ongoing research hotspots in molecular diagnostics‐target preamplification‐free detection, microRNA (miRNA) testing, non‐nucleic‐acid detection, and SARS‐CoV‐2 detection‐are also covered.
This review focuses on recent developments in both fundamental CRISPR biochemistry and CRISPR‐based molecular diagnostic technologies. Four emerging research topics on target preamplification‐free detection, microRNA (miRNA) detection, non‐nucleic‐acid detection, and SARS‐CoV‐2 detection are also covered. |
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ISSN: | 1433-7851 1521-3773 |
DOI: | 10.1002/anie.202214987 |