Insight into a Target-Induced Photocurrent-Polarity-Switching Photoelectrochemical Immunoassay for Ultrasensitive Detection of Escherichia coli O157:H7

• Published in: Analytical chemistry (Washington) 2023-02, Vol.95 (5), p.2698-2705
• Main Authors: Chen, Xiaomei, Yin, Mingming, Ge, Rui, Wei, Jie, Jiao, Tianhui, Chen, Qingmin, Oyama, Munetaka, Chen, Quansheng
• Format: Article
• Language: English
• Subjects: Antibodies; Biomolecules; Biosensing Techniques - methods; Copper oxides; E coli; Escherichia coli; Escherichia coli O157; Foodborne Diseases; Foodborne pathogens; Humans; Immunoassay; Immunoassay - methods; Immunosensors; Monoclonal antibodies; Near infrared radiation; Photoelectric effect; Photoelectric emission; Photoelectricity; Polarity; Portability; Smartphones
• ISSN: 0003-2700; 1520-6882
• DOI: 10.1021/acs.analchem.2c03235

Sensitive, portable methods of detection for foodborne pathogens hold great significance for the early warning and prevention of foodborne diseases and environmental pollution. Restricted by a complicated matrix and limited signaling strategies, developing a ready-to-use sensing platform with ultrahigh sensitivity remains challenging. In this work, near-infrared (NIR) light-responsive AgBiS2 nanoflowers (NFs) and Cu2O nanocubes (NCs) were introduced to construct a novel target-induced photocurrent-polarity-switchable system and verified for the development of an all-in-one, ready-to-use photoelectrochemical (PEC) immunosensor. NIR-responsive n-type AgBiS2 NFs and p-type Cu2O NCs producing anodic and cathodic photocurrents were conjugated with monoclonal (MAb1) and polyclonal antibodies (PAb2), respectively. Using a sandwich-type immunocomplex bridged by Escherichia coli O157:H7, an efficient photocurrent-polarity-switching PEC system was formed on a paper-based working electrode (PWE). Owing to the spatial separation of the photogenerated carriers and the elimination of false-positive/negative signals by the polarity-switchable photocurrent, the proposed NIR PEC immunoassay for E. coli O157:H7 exhibits a considerably low detection limit of 8 colony-forming units/milliliter (CFU/mL) with a linear range from 25 to 5 × 107 CFU/mL. The platform includes a PWE with an automatic cleaning function and a portable PEC analyzer with smartphone-compatible Bluetooth capability, thus achieving point-of-care testing of E. coli O157:H7. The sensor was applied to the analysis of pork samples artificially contaminated with E. coli O157:H7, and the detection results were in good agreement with the plate counting method, a gold standard in the field. This work aimed to investigate the photoelectric activity of the NIR-responsive p/n-type composites and to provide a new signal-reversal route for the construction of an all-in-one ready-to-use PEC immunosensor for the detection of low-concentration biomolecules.