Structural Changes in the Liver in Post-Toxic Cirrhosis and Its Treatment with Oxidized Dextran. Immunohistochemical Research

In adult Wistar rats, post-toxic liver cirrhosis was induced by intraperitoneal injection of 50% oil solution of CCl 4 and peroral administration of 6.5% aqueous solution of ethyl alcohol over 60 days. Histological examination of the liver revealed vacuolar degeneration and necrosis of hepatocytes,...

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Bibliographic Details
Published in:Bulletin of experimental biology and medicine 2023, Vol.174 (3), p.395-398
Main Authors: Karpov, M. A., Klochin, V. D., Nadeev, A. P., Shkurupy, V. A., Marinkin, I. O.
Format: Article
Language:English
Subjects:
Aldehydes
Animals
Apoptosis
Aqueous solutions
Ascites
Biomedical and Life Sciences
Biomedicine
Carbon tetrachloride
Carbon Tetrachloride - toxicity
Cell Biology
Cirrhosis
Collagen
Collagen - metabolism
Collagen Type I - genetics
Collagen Type I - metabolism
Degeneration
Dextran
Dextrans - metabolism
Fibroblasts
Hepatocytes
Immunohistochemistry
Internal Medicine
Laboratory Medicine
Liver
Liver - metabolism
Liver cirrhosis
Liver Cirrhosis - chemically induced
Liver Cirrhosis - drug therapy
Liver Cirrhosis - metabolism
Molecular weight
Pathology
Rats
Rats, Wistar
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Summary:In adult Wistar rats, post-toxic liver cirrhosis was induced by intraperitoneal injection of 50% oil solution of CCl 4 and peroral administration of 6.5% aqueous solution of ethyl alcohol over 60 days. Histological examination of the liver revealed vacuolar degeneration and necrosis of hepatocytes, formation of false lobules, expression of collagens I and III periportally and in interlobular spaces, ascites, and hydrothorax. Then, oxidized dextran with a molecular weight of 40 kDa (2 ml of a 5% aqueous solution) was intraperitoneally injected every fourth day over 30 days. Against the background of treatment with oxidized dextran, the volume density of collagens I and III decreased by more than 2 times, the “collagen-producing” activity of fibroblasts decreased by 47%, and the number of fibroblasts decreased, including by the mechanism of apoptosis. The decrease in the collagen content in the liver of rats treated with oxidized dextran was apparently associated with blockade of collagen assembly due to the aldehyde—aldehyde interaction of tropocollagens and oxidized dextran.
ISSN:0007-4888
1573-8221
DOI:10.1007/s10517-023-05715-3