A Complex of Type I Platelet-Activating Factor Acetylhydrolase (PAF-AH) Catalytic Subunits Switches from α1/α2 Heterodimer to α2/α2 Homodimer during Adipocyte Differentiation of 3T3-L1 Cells

• Published in: Biological & pharmaceutical bulletin 2023/02/01, Vol.46(2), pp.257-262
• Main Authors: Nakamura, Yasuhiro, Kihara-Negishi, Fumiko, Tanigawa, Kazunari, Kiriya, Mitsuo, Kadowaki, Yuki, Imagawa, Haruka, Nakanishi, Hiroki, Watanabe, Shigekazu, Maruyama, Keiji, Karasawa, Ken
• Format: Article
• Language: English
• Subjects: 1-Alkyl-2-acetylglycerophosphocholine esterase; 1-Alkyl-2-acetylglycerophosphocholine Esterase - genetics; 3T3-L1; 3T3-L1 Cells; Acetylcholinesterase; adipocyte; Adipocytes; Adipose tissue; Animals; Body weight; Catalytic Domain; Catalytic subunits; Cell Differentiation; differentiation; Food intake; Immunoprecipitation; Isoforms; Male; Mice; Phospholipases A - metabolism; Platelet Activating Factor - metabolism; Platelet-activating factor; Platelet-activating factor acetylhydrolase; platelet-activating factor acetylhydrolase (PAF-AH); Preadipocytes; Regulatory subunits; white adipose tissue (WAT)
• ISSN: 0918-6158; 1347-5215
• DOI: 10.1248/bpb.b22-00666

Platelet-activating factor acetylhydrolase (PAF-AH) hydrolyzes an acetyl ester at the sn-2 position of platelet-activating factor (PAF), thereby mediating a variety of biological functions. PAF-AH is found in three isoforms: Type I PAF-AH (PAF-AH I) and Type II PAF-AH (PAF-AH II) are intracellular enzymes whereas plasma PAF-AH is characterized by association with lipoprotein in plasma. PAF-AH I forms a tetramer constituted by two catalytic subunits (α1 and α2) with β regulatory subunits. We recently showed that a deficiency of PAF-AH I catalytic subunits in male mice caused an increase of body weight, food intake, and white adipose tissue (WAT) weight. In this study, we examined whether the expression of this enzyme was altered in the differentiation of 3T3-L1 preadipocytes into adipocytes. The amount of PAF-AH I α1 subunit protein was significantly reduced in 3T3-L1 differentiation, while the amount of the PAF-AH I α2 subunit was not changed. Immunoprecipitation analysis of 3T3-L1 differentiation showed that the complex of PAF-AH I catalytic subunits was changed from α1/α2 heterodimer to α2/α2 homodimer. Our findings suggest that changes in PAF-AH I catalytic subunits are involved in adipocyte differentiation of 3T3-L1 and obesity in mice.