Screen printed electrodes on interfacial Pt–CuO/carbon nanofiber functional ink for real-time qualification of cell released hydrogen peroxide

• Published in: Analytica chimica acta 2023-03, Vol.1245, p.340829-340829, Article 340829
• Main Authors: Zhang, Ning, Tong, Mengqi, Shi, Zhuanzhuan, Yang, Jianyu, Chen, Bo, Li, Changming, Guo, Chunxian
• Format: Article
• Language: English
• Subjects: Biosensing Techniques - methods; Carbon - chemistry; Carbon nanofiber; Electrodes; Functional ink; Hydrogen Peroxide; Ink; Interface engineering; Live-cell assay; Nanofibers - chemistry; Nanozyme; Screen printed electrode
• ISSN: 0003-2670; 1873-4324
• DOI: 10.1016/j.aca.2023.340829

Screen printed electrode (SPE) on carbon-based inks exhibits promising applications in biosensing, environment protection and food safety. We report here a unique carbon-based material comprising Pt–CuO nanocrystal interfacially anchored on functionalized carbon nanofiber (Pt–CuO@FCNF) and its functional ink to build SPE for ultrasensitive detection of cell released H2O2. Pt–CuO@FCNF is fabricated using a one-pot and mass production method through direct pyrolysis of Pt and CuO precursors together with FCNF. FCNF with 1-D structure and high electrical conductivity can interfically anchor Pt–CuO nanocrystal, which synergically promotes rich active site and catalytic activity towards H2O2. Pt–CuO@FCNF exhibits a wide linear response of 0.4 μM–11 mM, a low detection limit of 17 nM, a fast response time of 1.0 s, and good selectivity. Eventually, Pt–CuO@FCNF SPE realizes real-time and ultrasensitive qualification of H2O2 released from both normal and cancer cells. We report a unique carbon-based material comprising Pt–CuO nanocrystal interfacially anchored on functionalized carbon nanofiber (Pt–CuO@FCNF) and its screening printing electrode (SPE), realizing real-time qualification of H2O2 in live-cell assays. [Display omitted] •A unique carbon-based sensing material is produced by a one-pot and mass production method.•FCNF with 1-D structure and high conductivity interfacially anchors on Pt–CuO nanocrystal.•Pt–CuO@FCNF delivers wide linear range, low detection limit and fast response towards H2O2.•Pt–CuO@FCNF SPE achieves real-time and sensitive qualification of H2O2 released from cells.•Pt–CuO@FCNF could be extended to explore H2O2-related disease diagnosis and treatment.