Pectin decomposition at the early stage of brown-rot decay by Fomitopsis palustris

ABSTRACT The sapwood of Japanese cedar (Cryptomeria japonica D. Don) was decayed by the brown-rot fungus Fomitopsis palustris under bright and dark conditions. Scanning electron microscopy revealed the presence of mycelia inside the wood even after 1 week from the start of fungal exposure. Moreover,...

Full description

Saved in:
Bibliographic Details
Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2023-04, Vol.87 (5), p.555-562
Main Authors: Tanaka, Yuki, Nezu, Ikumi, Aiso, Haruna, Fujie, Tomomi, Konno, Naotake, Suzuki, Tomohiro, Ishiguri, Futoshi, Habu, Naoto
Format: Article
Language:English
Subjects:
Coriolaceae
Fungal Proteins
Pectins
Wood - microbiology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:ABSTRACT The sapwood of Japanese cedar (Cryptomeria japonica D. Don) was decayed by the brown-rot fungus Fomitopsis palustris under bright and dark conditions. Scanning electron microscopy revealed the presence of mycelia inside the wood even after 1 week from the start of fungal exposure. Moreover, holes were observed in the torus after fungal exposure. Ruthenium red staining revealed that the pectin in pits was largely absent for 3 weeks. These events occurred before the mass loss of wood samples was confirmed at the early stage. Moreover, FpPG28A was more highly expressed at the hyphal front on a pectin-containing medium under dark conditions compared with bright conditions. This up-regulation under dark conditions indicated that the pectin decomposition ability was promoted inside the wood where light could not reach. In conclusion, we suggest that the brown-rot fungus completed its hyphal expansion within the wood via pectin decomposition in pits before holocellulose decomposition. Graphical Abstract Graphical Abstract The gene of FpPG28A (polygalacturonase) was up-regulated under dark conditions compared with bright conditions.
ISSN:1347-6947
1347-6947
DOI:10.1093/bbb/zbad014