Iron inactivation by Sporobolomyces ruberrimus and its potential role in plant metal stress protection. An in vitro study

The endophytic Basidiomycete Sporobolomyces ruberrimus protects its host Arabidopsis arenosa against metal toxicity. Plants inoculated with the fungus yielded more biomass and exhibited significantly fewer stress symptoms in medium mimicking mine dump conditions (medium supplemented with excess of F...

Full description

Saved in:
Bibliographic Details
Published in:The Science of the total environment 2023-04, Vol.870, p.161887-161887, Article 161887
Main Authors: Jędrzejczyk, Roman J., Gustab, Maciej, Ważny, Rafał, Domka, Agnieszka, Jodłowski, Przemysław J., Sitarz, Maciej, Bezkosty, Patryk, Kowalski, Michał, Pawcenis, Dominika, Jarosz, Kinga, Sebastian, Victor, Łabaj, Paweł P., Rozpądek, Piotr
Format: Article
Language:English
Subjects:
Arabidopsis
Arabidopsis arenosa
Basidiomycota
Iron - chemistry
Iron - toxicity
iron inactivation
Metals
Spectroscopy, Fourier Transform Infrared
Toxic metals
X-Ray Diffraction
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The endophytic Basidiomycete Sporobolomyces ruberrimus protects its host Arabidopsis arenosa against metal toxicity. Plants inoculated with the fungus yielded more biomass and exhibited significantly fewer stress symptoms in medium mimicking mine dump conditions (medium supplemented with excess of Fe, Zn and Cd). Aside from fine-tuning plant metal homeostasis, the fungus was capable of precipitating Fe in the medium, most likely limiting host exposure to metal toxicity. The precipitated residue was identified by Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), X-Ray Diffraction (XRD) and electron microscopy (SEM/TEM) with energy dispersive X-Ray analysis (EDX/SAED) techniques. The performed analyses revealed that the fungus transforms iron into amorphous (oxy)hydroxides and phosphates and immobilizes them in the form of a precipitate changing Fe behaviour in the MSR medium. Moreover, the complexation of free Fe ions by fungi could be obtained by biomolecules such as lipids, proteins, or biosynthesized redox-active molecules. [Display omitted] •S. ruberrimus facilitates A. arenosa growth in sterile metal enriched medium.•S. ruberrimus is capable of precipitating Fe deposits.•A. arenosa cocultered with the yeast for 10 days exhibited less stress symptoms.
ISSN:0048-9697
1879-1026
DOI:10.1016/j.scitotenv.2023.161887