Calotropis procera latex protein reduces inflammation and bone loss in ligature-induced period ontitis in male rats

• Published in: Archives of oral biology 2023-03, Vol.147, p.105613-105613, Article 105613
• Main Authors: Melo, Iracema Matos, Sarte, Marina Fiuza, Tavares, Samia Jéssica Silva, Lustosa, Maria Socorro, Oliveira, Jefferson Soares, Alencar, Nylane Maria Nunes, Ramos, Márcio Viana, Lima, Vilma
• Format: Article
• Language: English
• Subjects: Alveolar Bone Loss - pathology; Alveolar Process - metabolism; Animals; Antioxidants; Calotropis - metabolism; Inflammation - prevention & control; Interleukin-1 beta; Latex - pharmacology; Male; Neutrophil; Osteoprotegerin; Osteoprotegerin - pharmacology; Periodontal disease; Peroxidase; RANK Ligand - metabolism; Rats; Rats, Wistar; Receptor activator of NF-κB ligand; Tartrate-Resistant Acid Phosphatase
• ISSN: 0003-9969; 1879-1506
• DOI: 10.1016/j.archoralbio.2023.105613

Calotropis procera latex protein (CpLP) is a popular anti-inflammatory and therefore we aimed to study its effects on inflammatory bone loss. Male Wistar rats were subjected to a ligature of molars. Groups of rats received intraperitoneally CpLP (0.3 mg/kg, 1 mg/kg, or 3 mg/kg) or saline (0.9% NaCl) one hour before ligature and then daily up to 11 days, compared to naïve. Gingiva was evaluated by myeloperoxidase activity and interleukin-1 beta (IL-1β) expression by ELISA. Bone resorption was evaluated in the region between the cement-enamel junction and the alveolar bone crest. The histology considered alveolar bone resorption and cementum integrity, leukocyte infiltration, and attachment level, followed by immunohistochemistry bone markers between 1st and 2nd molars. Systemically, the weight of the body and organs, and a leukogram were performed. The periodontitis significantly increased myeloperoxidase activity and the IL-1β level. The increased bone resorption was histologically corroborated by periodontal destruction, leukocyte influx, and attachment loss, as well as the increasing receptor activator of the nuclear factor-kappa B ligand (RANKL)/osteoprotegerin (OPG) ratio, and Tartrate-resistant acid phosphatase (TRAP)+ cells when compared to naïve. CpLP significantly reduced myeloperoxidase activity, level of IL-1β, alveolar bone resorption, periodontal destruction, leukocyte influx, and attachment loss. The CpLp also reduced the RANKL/OPG ratio and TRAP+ cells, when compared with the saline group, and did not affect the systemic parameters. CpLP exhibited a periodontal protective effect by reducing inflammation and restricting osteoclastic alveolar bone resorption in this rat model. [Display omitted]