Determination of Bacterial Diversity of Propolis Microbiota

Propolis is a natural resinous mixture produced by the excretions of honeybees. PCR amplification of the 16S rRNA gene region was achieved using DNA of pre‐enriched propolis samples collected from Apis mellifera production hives (n=37) in Eastern Türkiye (Bingöl and its regions). Next‐generation seq...

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Bibliographic Details
Published in:Chemistry & biodiversity 2023-03, Vol.20 (3), p.e202201182-n/a
Main Authors: Ersoy Omeroglu, Esra, Keriman Arserim‐Uçar, Dılhun, Yegin, Zeynep, Çağlayan, Nevzat, Nur Zafer Yurt, Mediha, Busra Tasbasi, Behiye, Esma Acar, Elif, Ucak, Samet, Cengiz Ozalp, Veli, Sudagidan, Mert
Format: Article
Language:English
Subjects:
Animals
Apis mellifera
Ascomycota
Bacteria
Bees
Deoxyribonucleic acid
DNA
Firmicutes
Humans
metabarcoding
Microbiota
next generation sequencing
Propolis
Resins
RNA, Ribosomal, 16S - genetics
rRNA 16S
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Summary:Propolis is a natural resinous mixture produced by the excretions of honeybees. PCR amplification of the 16S rRNA gene region was achieved using DNA of pre‐enriched propolis samples collected from Apis mellifera production hives (n=37) in Eastern Türkiye (Bingöl and its regions). Next‐generation sequencing and metabarcoding techniques were used to identify bacterial communities in propolis samples. Firmicutes dominated the phylum structure, with Proteobacteria, Actinobacteria, Tenericutes, and Spirochaetes following. The top three bacterial families were Bacillaceae, Enterobacteriaceae, and Enterococcaceae. Bacillus (dominantly B. badius and B. thermolactis at the species level) was recognized at the genus level, followed by Enterococcus and Clostridium sensu stricto. Our study comprehensively identified the bacterial diversity of propolis samples. Further investigations targeting to enlighten the microbiota of propolis and its potential application fields are required to gain better insight into ecological, nutritional, and medicinal perspectives.
ISSN:1612-1872
1612-1880
DOI:10.1002/cbdv.202201182