Promising antimicrobial and antibiofilm activities of Orobanche aegyptiaca extract-mediated bimetallic silver-selenium nanoparticles synthesis: Effect of UV-exposure, bacterial membrane leakage reaction mechanism, and kinetic study

• Published in: Archives of biochemistry and biophysics 2023-03, Vol.736, p.109539-109539, Article 109539
• Main Authors: Mostafa, Hamida Y., El-Sayyad, Gharieb S., Nada, Hanady G., Ellethy, Rania A., Zaki, E.G.
• Format: Article
• Language: English
• Subjects: Anti-Bacterial Agents - metabolism; Anti-Bacterial Agents - pharmacology; Antibiofilm; Antimicrobial activity; Bacteria; Bimetallic nanoparticles; Biofilms; Escherichia coli - metabolism; Metal Nanoparticles; Microbial Sensitivity Tests; Orobanche - metabolism; Orobanche aegyptiaca extract; Plant Extracts - metabolism; Plant Extracts - pharmacology; Selenium - pharmacology; Silver - pharmacology; Staphylococcus aureus; Staphylococcus aureus - metabolism; Ultraviolet Rays
• ISSN: 0003-9861; 1096-0384
• DOI: 10.1016/j.abb.2023.109539

In this research, Orobanche aegyptiaca extract was utilized as an eco-friendly, and cost-effective green route for the construction of bimetallic silver-selenium nanoparticles (Ag-Se NPs). Bimetallic Ag-Se NPs were characterized by XRD, EDX, FTIR, HR-TEM, DLS, SEM/mapping and EDX studies. Antimicrobial, and antibiofilm potentials were tested against some selected pathogenic bacteria and unicellular fungi by ZOI, MIC, effect of UV exposure, and inhibition %. Reaction mechanism was assessed through membrane leakage assay and SEM imaging. HRTEM analysis confirmed the spherical nature and was ranged from 18.1 nm to 72.0 nm, and the avarage particle size is determined to be 30.58 nm. SEM imaging prove that bimetallic Ag-Se NPs presents as a bright particles, and both Ag and Se were distributed equally across O. aegyptiaca extract and Guar gum stabilizers. ZOI results showed that, bimetallic Ag-Se NPs have antimicrobial activity against S. aureus (20.0 nm), E. coli (18.5 nm), P. aeruginosa (12.6 nm), and C. albicans (18.2 nm). In addition, bimetallic Ag-Se NPs were able to inhibit the biofilm formation for S. aureus by 79.48%, for E. coli by 78.79%, for P. aeruginosa by 77.50%, and for C. albicans by 73.73%. Bimetallic Ag-Se NPs are an excellent disinfectant once it had excited by UV light. It was observed that the quantity of cellular protein discharged from S. aureus is directly proportional to the concentration of bimetallic Ag-Se NPs and found to be 244.21 μg/mL after the treatment with 1 mg/mL, which proves the antibacterial characteristics, and explains the creation of holes in the cell membrane of S. aureus producing in the oozing out of the proteins from the S. aureus cytoplasm. Based on the promising properties, they showed superior antimicrobial potential at low concentration (to avoid toxicity) and continued-phase durability, they may use in pharmaceutical and biomedical applications. [Display omitted] •Eco-friendly, and cost-effective green synthesis of bimetallic Ag-Se NPs using Orobanche aegyptiaca extract.•Ag-Se NPs were characterized by XRD, EDX, FTIR, HR-TEM, DLS, SEM/mapping and EDX analysis.•Ag-Se NPs showed superior antimicrobial potential at low concentration (to avoid toxicity) and continued-phase durability.•Ag-Se NPs performs a noted spots around the tested pathogenic bacteria, and confirmed the bleeding out of protein molecules from the membrane of bacterial cell.