Effects of different coating materials on the morphological characteristics of chicken adenohypophyseal folliculo‐stellate cells in vitro

• Published in: Animal science journal 2023-01, Vol.94 (1), p.e13814-n/a
• Main Authors: Nishimura, Shotaro, Yamahira, Shungo, Chowdhury, Vishwajit S., Hosaka, Yoshinao Z.
• Format: Article
• Language: English
• Subjects: adenohypophysis; Animals; Antibodies; Cell culture; Cell proliferation; Cells, Cultured; chicken; Chickens; Chickens - metabolism; Coating; Coatings; Collagen; collagen coating; Collagenase; Cytokeratin; Cytology; Endocrine Cells - metabolism; folliculo‐stellate cells; Growth hormones; Immunocytochemistry; Laminin; Luteinizing hormone; Lysine; Morphology; Physical characteristics; Plates; Poultry; Prolactin; Prolactin - metabolism; Protective coatings; S100b protein; Stellate cells; Trypsin
• ISSN: 1344-3941; 1740-0929
• DOI: 10.1111/asj.13814

Chicken adenohypophyseal cells were cultured in plates coated with different materials, and their morphologies were examined to confirm the characteristics of chicken folliculo‐stellate (FS) cells in vitro. The adenohypophyseal cells were dispersed with a collagenase/trypsin mixture in media and seeded in plates coated in either poly L‐lysine (PLL), collagen, or laminin. After 7 days of culture, the cells were fixed and immunocytochemistry was performed. 5‐Bromo‐2′‐deoxyuridine incorporation test indicated that the proliferation activity of the culture cells was different based on the coating materials, and it was higher in the collagen‐coated plate than two other coating materials. Fluorescence immunocytochemistry was also performed using mixed antibodies against growth hormone, prolactin, luteinizing hormone β‐subunit, basic cytokeratin (bCK), and S100B. The culture cells on the PLL‐ and laminin‐coated surfaces were round or oval in shape, and bCK‐immunopositive FS cells were morphologically indistinguishable from endocrine cells. In the collagen‐coated plate, many endocrine cells were round or oval in shape, but FS cells displayed a larger and flattened morphology. S100B‐immunoreactions were localized in the nuclei of bCK‐immunopositive FS cells. These results suggest that culturing the chicken adenohypophyseal cells in the collagen‐coated plate enables the distinction of FS cells from endocrine cells.