Anti-tumor effect of infant-derived Enterococcus via the inhibition of proliferation and inflammation as well as the promotion of apoptosis

Probiotic WEHI01 and WEFA23 from infants were previously found to effectively inhibit the development of melanoma. In this study, their immunomodulatory and antitumor mechanisms were systemically studied. assay showed that WEHI01 and WEFA23 achieved biphasic immune regulation, which was revealed by...

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Bibliographic Details
Published in:Food & function 2023-02, Vol.14 (4), p.2223-2238
Main Authors: Yang, Qin, He, Yao, Tian, Linlin, Zhang, Zhihong, Qiu, Liang, Tao, Xueying, Wei, Hua
Format: Article
Language:English
Subjects:
Animals
Anticancer properties
Antitumor activity
Apoptosis
Bcl-2 protein
Caspase-3
Cell activation
Cell Proliferation
Enterococcus
Enterococcus faecium - physiology
Gelatinase A
Gelatinase B
Hypoxia-inducible factor 1a
Immune system
Immunomodulation
Immunoregulation
Infants
Inflammation
Interleukin 17
Interleukin 6
Leukocyte migration
Lymphocytes
Macrophages
Melanoma
Mice
Spleen
Transforming growth factor-b
Tumors
Vascular endothelial growth factor
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Summary:Probiotic WEHI01 and WEFA23 from infants were previously found to effectively inhibit the development of melanoma. In this study, their immunomodulatory and antitumor mechanisms were systemically studied. assay showed that WEHI01 and WEFA23 achieved biphasic immune regulation, which was revealed by the activation of resting spleen lymphocytes and RAW264.7 macrophages, as well as the anti-inflammation effect when immune cells were treated with LPS. The antitumor effects of WEHI01 and WEFA23 and were then investigated. CCK8 and the cell scratch assay showed that the conditioned media, which were co-incubated with and spleen lymphocytes, significantly inhibited the proliferation and migration of B16F10, HepG-2 and HT-29 cells. The results of the tumor-bearing mice model experiment showed that WEFA23 inhibition of the growth of tumors in mice, and the anti-tumor mechanism involved three aspects, namely tumor proliferation (decreasing expressions of LDHA, VEGF, MMP2, MMP9 and HIF-1α), inhibition of the pro-inflammation state (decreasing expressions of IL-6, TGF-β and IL-17) and the promotion of apoptosis (increasing expression of Bax/Bcl-2, caspase-3 and p53). The results suggest that the two strains of could be promising candidates for treating melanoma with a highly inhibitory effect.
ISSN:2042-6496
2042-650X
DOI:10.1039/d2fo03045d